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Genetic Mapping and Functional Analyses of Arabidopsis Male Sterile Mutant ms1142

Author: ChangYuHua
Tutor: ZhangSen；YangZhongNan
School: Shanghai Normal University
Course: Biochemistry and Molecular Biology
Keywords: Arabidopsis Male sterility Microspore Sporopollenin Pollen exine
CLC: Q943
Type: Master's thesis
Year: 2010
Downloads: 52
Quote: 0
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Abstract


ms1142 is a male sterile mutant EMS mutagenesis of wild - type Arabidopsis groups screened . Short , ms1142 mutant compared with the wild-type pods , excluding seeds . Genetic analysis showed that the mutant phenotype for a single recessive nuclear gene mutation . Map-based cloning method MS1142 gene is located on the the Arabidopsis genome chromosomes BAC clone F16P17 on 44kb interval . Bioinformatics analysis predicted that the range of genes and DNA sequencing , found that a gene (AT1G62940) gave birth to a single base change , resulting in a protein that the gene 232 amino acid proline change leucine . The genetic complementation experiments and allelic analysis of experimental results confirmed the gene mutation leads ms1142 mutant male sterility . Histological examination was found that the In anther development process , starting from the eighth issue , mutant spores began to break down , and finally mature pollen sacs full of pollen grains , only some debris . The scanning electron microscopy results also show that the the mutant anthers small and medium-sized spores outer wall abnormalities and rupture . Moreover , transmission electron microscopy analysis showed that small spores can not be normal deposition the sporopollenin thus rules the outer wall structure . Real-time PCR and double mutant experimental results show that the downstream the MS1142 gene action in the AMS , and in situ hybridization experiment results show that the the MS1142 gene and the AMS gene between upstream and downstream indirect relationship . These results suggest that the MS1142 gene formation plays an important role in the development of small spores and pollen outer wall .

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CLC: > Biological Sciences > Botany > Plant Cell Genetics
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