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The Experimental Study on the Alteration of Substance P on the Airway Inflammation in Allergic Rhinits Model in Rats

Author: HanXiaoBo
Tutor: GuanZuo
School: China Medical University
Course: Otorhinolaryngology
Keywords: Rhinitis allergic perennial allergic Airway Inflammation substance P Bronchoalveolar lavage fluid Interleukin-4
CLC: R765.21
Type: Master's thesis
Year: 2010
Downloads: 28
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Abstract


ObjectiveObserve the pathologic histology and the expression of substance P(SP) of nasal mucosa and lung tissue in allergic rhinits model in Rats.Determine the level of the Interleukin-4 in BALF at the same time. To explore the relationship and possible mechanism on airway inflammations of allergic rhinitis model in rats.MethodsTwenty-four Wistar rats were randomly divided into two experimental groups (12 in each):normal control group and allergic rhinitis model group.The allergic rhinitis models of rats were established by intraperitoneal injection of ovalbumin(OVA) and nasal challenge with OVA.Hematoxylin-eosin(HE) staining was used to observe the pathologic histology of nasal mucosa and lung tissue. The total Leucocyte numbers and differentiated cell counts in bronchial alveolus lavage fluid (BALF) were performed. The level of the Interleukin-4 (IL-4) in BALF was determined by using enzyme-linked immunosorbent assay (ELISA). The expression of substance P(SP) in nasal mucosa and bronchial tissue was observed with immunohistochemical techniques. Use the computer image disposition system to make semiquantitative analysis of the area of positive cell.ResultsIn allergic rhinitis model group, the infiltration of inflammatory cells were observed in both nasal mucosa and lung. The results of semiquantitative analysis indicate that the expressions of SP in nasal mucosa and bronchia in allergic rhinitis model group were both higher than those in control group.The statistics analysis shows that the level of interleukin-4 in BALF were all obviously higher than those of normal control group (P＜0.05). In allergic rhinitis group, the expression of SP in nasal mucosa was a significant positive correlation with the expression of SP in bronchia (r=0.750;.P<0.05);There is a significant positive correlation between the concentration ofIL-4 in BALF and the expression of SP in bronchia (r=0.612; P＜0.05).ConclusionsAllergic inflammation in nasal mucosa leads to changes in both histopathology and immunology, and initiates the inflammation in lower respiratory tract. SP plays an important role in early allergic Airway Inflammation.

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CLC: > Medicine, health > Otorhinolaryngology > Rhinology,nasal disease > Nasal disease > Rhinitis
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