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Five pairs of primers were designed according to the entire gene sequences of Bovine Herpesvirus-1 in GenBank（NC001847）,and the cDNA of the five genes were amplified by PCR with the total extracted DNA of the IBRV NM06 isolate isolated in Inner Mongolia.The PCR products were ligated with PMD19-T vector and cloned,the recombinant plasmid obtained were certified by PCR.The results showed that the cDNA of gE gene was long 2086bp,which including a whole open reading frame,1728bp,which codes 576 amino acids;the cDNA of gI gene, 1280bp,also includes a whole open reading frame,1149bp,which codes 383 amino acids;the cDNA of gG gene,1559bp,also includes a whole open reading frame,1335bp,which codes 445 amino acids;and the cDNA of gN gene,1459bp,includes two whole open reading frames,the first open reading frame,291bp,which codes 97 amino acids;the second open reading frame,777bp,which codes 259 amino acids.Comparision of the homology among gE,gI,gG,gN,VP22 and accordingly gene of the reference strains,the results showed that the homology of nucleotide sequences and deduced amino acid sequences of gE gene among NM06 with other strains were 93.4%～99.9% and 83.8%～99.8%,respectively;The homology of nucleotide sequences and deduced amino acid sequences of gI gene among NM06 with other strains were 96.2%～99.8% and 89.2%～99.5%,respectively;The homology of nucleotide sequences and deduced amino acid sequences of gG gene among NM06 with other strains were 97.4%～99.8% and 95.9%～99.5%,respectively;The homology of nucleotide sequences and deduced amino acid sequences of gN gene among NM06 with other strains were 99.3% and 97.9%,respectively;The homology of nucleotide sequences and deduced amino acid sequences of VP22 gene among NM06 with other strains were 100% and 99.6%～100%,respectively.The homology of nucleotide sequences and deduced amino acid sequences of five genes among NM06 with accordingly BHV-5 gene were 71.7%～82.9% and 69.5%～82.6%,respectively. Construction of phylogenetic tree analysis showed that NM06 isolate with the can kao and cooper strains which isolated in Sweden were in the same evolutionary branch,but NM06 isolate with BHV-5 and CvHV-2 were in the different evolutionary branch.These results suggested that IBRV NM06 isolate has a close relation to Sweden type.IBRV NM06 isolate belongs to Bovine Herpesvirus type-1（1.1）,according to homology analysis and phylogenetic tree analysis.
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