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Pepper (Capsicum annuum L.) is a very important vegetable crops, heterosis, cytoplasmic male sterility pepper Hybridizing. Pepper male sterile line 8214A and its corresponding retention system the 8214B test materials, ultrastructure and starch grains from the anther development characteristics of the distribution of cell biology, comparative study, the results are as follows: 1, infertility Department of the ultrastructure and maintain Anther Anther Development process. Maintain the Department of pollen mother cell stage, the mother cell nucleus, ranking central cell cytoplasm strong, when the anther wall differentiated; mother cells during meiosis, the mother cell volume increases, disappear with the nucleus to form a clear and legible the chromosome, when the anther wall the tapetum cell volume radial increase, the organelles become rich; tetrad tetrad microspore cytoplasm strong, rich organelles have begun to form pollen exine, the tapetum the rules of the layer of cells, organelles rich in sporopollenin rely on the the medicine room side of the wall of the accumulated; in microspore early, microspore nucleus is located in the Central At this time the the tapetum cell shape becomes irregular, is beginning to show signs of deterioration ; Subsequently, the formation of many small vacuoles in the microspore cytoplasm, small vacuoles fuse to form a large vacuole, into the late microspore when the anther wall tapetum cells completely degraded, leaving only remnants; microspore then completed the first mitosis form vegetative cells contain large vacuoles and was convex lens-shaped germ cell cellular early pollen; the two cellular late and mature pollen stage, with the disappearance of large vacuoles of vegetative cells, accumulate large amounts of starch grains in the pollen cytoplasm, when endothecium cell the anther wall only remaining epidermal cells and cell wall thickening. CMS in the period of the mother cell ultrastructure of anther maintain system similar; mother cell meiosis Ⅰ times during the dyad formed after the split, the dyad spore membrane localized shrinkage and rupture, containing exudation, when the anther wall tapetum cells showed signs of significant degradation, irregular cell shape, cell electron density increases, cell vacuolization, the nucleus is incomplete, there are many nucleosomes; complete meiosis. drug chamber volume increases are not sticking together formed tetrad microspore tetrad abnormal membrane local contraction and rupture, cytoplasmic local apparent contraction, no pollen exine formation, when the tapetum completely degraded; subsequently tetrad the small spores nucleus shape becomes irregular and the formation of many of the nucleosome cytoplasm further contraction last tetrad microspores completely degraded degradation, leaving only callose shell; anther pollen empty flat in the mature stage, . 2, Anther Development process cytologic features of the distribution of starch granules sterile line and keep PAS reaction. Before meiosis, keep the ANTHERS and Sterile anther structure and starch grain distribution is similar. Keep the ANTHERS meiosis, anther wall tapetum cells vacuolated volume increased accumulation of many smaller starch grains in parenchyma cells; late microspores, tapetum cell degeneration in the the parenchyma cells starch grains volume increases; cellular pollen period, with the disappearance of pollen in the pollen vacuole starch grains; pollen maturation, accumulated in their cytoplasm rich in starch grains. The sterile line anther meiosis, the drug chamber cavity space can not expand tetrad is squeezed together, the final tetrad microspore abortion. Normal the sterile anthers vascular tissue development, but more starch grains accumulated in the parenchyma cells. The kinds of chili male sterile line, the pollen abortion occurred in the tetrad stage. The tapetum cell structure abnormal carbohydrate may affect the normal transit to the drug chamber. The Male Sterility Line tapetal abnormalities and pollen sterility.
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