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Grape powdery mildew Uncinula necator (Schw.), Burr is a fungal diseases all over the world grape production producing areas around the world, grape cultivation, production have different levels of hazards. Therefore, the use of the the wild grapes resources to powdery mildew, for grape powdery mildew interaction the grape differences in the relationship of powdery mildew induced gene expression, screening of powdery mildew resistance genes will be an effective way to foster resistance to powdery mildew of grape varieties world. The study is based on high resistance to powdery mildew in the research group of Chinese Wild Vitis -24, the use of suppression subtractive hybridization (Suppression subtractive hybridization, SSH) technology to build Vitis -24 inoculated with powdery mildew, bacteria and unvaccinated under SSH library, sequence analysis filter out high resistance to host-specific expression of gene fragments obtained the following major findings: 1, high resistance to grape powdery mildew Chinese Wild Vitis suppliers -24 as the materials were extracted with the improved SDS / phenol method vaccination and inoculation in leaves total RNA and mRNA isolated from unvaccinated powdery mildew on the basis of the respective mRNA to gross grape leaves as the control side (Driver), mRNA leaves inoculated with powdery mildew Vitis test side (Tester), using suppression subtractive hybridization (suppressive subtractive Hybridization, SSH) to build a high resistance to powdery mildew powdery mildew induced Vitis -24 blades SSH-cDNA library. Randomly selected SSH library of 516 positive clones were sequenced, a total of 502 high-quality of ESTs, remove the vector sequence and the linker sequence fragment size range of 250-800bp. 502 sequences obtained by Blastx and Blastn ratio analysis showed 19 matching sequences in GenBank, 3.7% of the total sequence; 483 sequences with known gene sequences highly homologous, 281 known functional gene sequence performance of the high degree of homology, representing 54.5% of the total sequence, 202 fake protein and unnamed high sequence homology 39.1% of the total sequence. 281 EST analysis library known, their specific functions are classified as follows 10: the active oxygen scavenging, accounting for 5.0% of the known functional gene sequences, protein transport was 12.1%, the defense response 18.9%, involved in signal transduction of 5.3%, 30% of the energy metabolism, the secondary metabolites 9.3%, 7.1% transcriptional regulation, protein together on behalf of the metabolism of 9.6%, 2.1% cell structure, osmotic adjustment was 0.7%. 4, obtained Resistance-related Genes: flavonol synthase, pathogenesis-related protein, chitinase, chalcone synthase, serine / threonine protein kinase, shaggy-related protein kinase, protein kinase signal interaction 02, colorless anthocyanidin reductase, cysteine ??protease, metal S protein, catalase, glutamine synthetase, glycolic acid oxidase, proline rich protein, calmodulin, DnaJ-like protein F-box family protein, drought-induced protein, senescence-associated protein, lipid transfer protein.
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