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For understand the color potato bottom Xirui , Lasseter -01 , Black Beauty , Green potato 168, astringent skin red, washed red varieties in cytogenetics and DNA molecular level genetic differences in color potato germplasm identification and cross breeding. provide the basis for the test using conventional chromosome preparation methods and ISSR molecular marker technology its karyotype and polymorphism analysis . The results showed that : ① colored potato varieties RTC chromosome numbers are 48 , is a tetraploid ( 2n = 4x = 48 ) . The ② varieties the bottom Xirui , Black Beauty karyotype formulas are 2n = 4x = 48 = 36m 12sm, The Lasseter -01 to 2n = 4x = 48 = 32m 16sm, Qingshu 168 2n = 4x = 48 = 24m 24sm , astringent skin red 2n = 4x = 48 = 44m 4sm, the washed red 2n = 4x = 48 = 40m 8sm . The ③ the the varieties bottom Xirui , Black Beauty , astringent skin belongs to 1B karyotype the Lasseter -01, Qingshu 168 washing the red belongs 2B karyotype . ④ 12 ISSR suitable primer of of six colored potato varieties genome DNA amplification legible bands 151 , 136 polymorphic bands , percentage of polymorphic bands was 88.2% , rich genetic variation , ISSR fingerprinting the map can be used as the molecular basis of recognition between the six colored potato varieties . ⑤ colored potato varieties genetic distance (GD) between 0.4803 to 0.7073 , GD value of 0.57 , which is divided into three categories : first base Xirui , Black Beauty , and the second category is the Qarase special -01 , astringent skin , washed red , green potato 168 separate a class .
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