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Characterization of Chitin Metabolism Related Genes in Puccinia Striiformis F.Sp. Tritici and Sequence Analysis of Fungal Polysaccharide Deacetylase Genes

Author: LiangXiaoFei
Tutor: KangZhenSheng
School: Northwest University of Science and Technology
Course: Plant Pathology
Keywords: Wheat stripe rust Chitin Polysaccharides deacetylase Evolutionary analysis
CLC: S435.121
Type: Master's thesis
Year: 2010
Downloads: 33
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Abstract


Chitin is an important structural component of the fungal cell wall, to its normal anabolic plant pathogenic fungi to maintain the integrity of the cell structure and pathogenicity important; studies have shown that the rust chitin deacetylase (Chitin Deacetylase, the CDA) may and pathogens to evade host recognition and defense reactions related. Genome to retrieve found some fungi there are a greater number of CDA like protein PDAs (Polysaccharide Deacetylase proteins), the sequence features similar to CDA, it is not clear correspondence between PDAs and CDAs, there is no of these PDAs system evolution analysis. In order to understand the chitin metabolism-related genes in the wheat stripe rust (Puccinia striiformis f. Sp. Tritici) role in the differentiation of the infection process, and fungi in PDAs basic characteristics of the study of wheat stripe rust II class several chitin synthase (Chitin synthase, Chs) gene PstChs II and several PDA gene PstPDAs cloning and expression analysis, prokaryotic expression and purification, which PstPDA1 and PstPDA2 two genes of 90 species of fungi six PDAs preliminary sequence analysis. Main results are as follows: 1. PstChs II and PstPDAs of genes encoding proteins having the characteristic motifs and domains of the corresponding protein. Of PstChs II gene the summer spore germination process upregulated speculated germ tube extends the process of cell wall chitin synthesis; the different PstPDA differences in gene expression characteristics, may function in different differentiation period; PstPDA1 gene transcription this, due to alternative splicing intron and wheat stripe rust urediniospores dual-core features; obtained PstPDA1, PstPDA2 gene prokaryotic expression protein. Corrected according to the characteristics of the protein sequence and intron splice site 90 PDAs in the fungal genome annotation of 22 members, 11 of the initial 18 excluding the signal peptide sequence re-annotation secretory protein. The preliminary analysis of the evolutionary characteristics of fungi PDAs. Species-specific gene duplication caused by wheat stem rust (Puccinia graminis) gray cover Coprinus (Coprinus cinera), PDAs gene family expansion rice Rhizopus (Rhizopus oryzae) species, three species were identified 13 species-specific gene replication group Coprinus (C. cinereus as), rice Rhizopus (R. oryzae) replication group members; gray cover key amino acid sites exist in PDAs conservative motifs diversity. Phylogenetic analysis of fungal PDAs divided into three subfamilies; motifs are not full members of the subfamily III; contains some of the members of the subfamily I the PDA structure extraterritorial other domains, Basidiomycotina PDAs DXXDW \point conservative is poor; subfamily II no fungal ascomycete subphylum members. 4 based on the comparison of the the PDA gene loci sequences identified two sets of sequence conservation supercontigs wheat stem rust (P. graminis). The wherein supercontig34 supercontig100 there are a total of at least 80 kb of the linear region, the region memory duplication and insertion of the DNA fragment.

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CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Crop pests and diseases and their prevention > Cereal crop pests and diseases > Wheat pests and diseases > Disease
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