Dissertation > Excellent graduate degree dissertation topics show

The Effects of Human Urinary Kallidinogenase on Focal Cerebral Ischemical Reperfusion Injury in Rats

Author: FanLei
Tutor: YangJinSheng
School: Lanzhou University
Course: Neurology
Keywords: Human urine kininogenase Apoptosis Caspase-3 AIF Survivin Cerebral ischemia and reperfusion Learning and Memory morris water maze
CLC: R743.3
Type: Master's thesis
Year: 2010
Downloads: 107
Quote: 0
Read: Download Dissertation

Abstract


The first part of the urine kinin the original enzyme neuronal apoptosis after cerebral ischemia reperfusion Objective: To observe the human urinary kallikrein (HUK) on cerebral ischemia-reperfusion (I / R) brain the ischemic cortex ischemic lesions surrounding area Caspase-3. apoptosis-inducing factor (AIF) and the time of Survivin expression and neuronal apoptosis. Methods: Wistar rats were randomly divided into sham group, model group and the the HUK intervention group, application suture method to establish the middle cerebral artery occlusion (MCAO) and reperfusion model. Sham group at postoperative model group, HUK intervention group in ischemia 2h reperfusion 6h, 24h, 48h, 72h, application of immunohistochemistry and in situ end labeling (TUNEL) method to detect phase ischemic lesions surrounding brain regions Caspase-3. AIF. Survivin and expression of TUNEL-positive cells. Results: rat I / R each time point in the surrounding area of ??the ischemic lesion can be seen the of Caspase-3.AIF.Survivin and the expression of TUNEL-positive cells, compared to the HUK group and the model group, two groups of Caspase-3.AIF The same trend of Survivin and expression of TUNEL-positive cells the of caspase-3.AIF and TUNEL expression of the peak in the 24 hours after I / R, Survivin expression peak at 48 h after I / R. The HUK group at each time point of Caspase-3, AIF and TUNEL-positive cells in the optical density value compared with the model group significantly reduced of Survivin optical density values ??compared with the model group was significantly higher (all P lt; 0.05) Conclusion: HUK rats cerebral ischemia reperfusion injury has a protective effect and its mechanism of action may inhibit the expression of Caspase-3.AIF with HUK promote expression of Survivin, reduce delayed neuronal apoptosis. Keywords: human urine kinin original enzyme; apoptosis; Caspase-3; AIF; of Survivin; cerebral ischemia reperfusion in the second part of the human urinary kinin the original enzyme on cerebral ischemia-reperfusion spatial learning and memory function and cortical nestin expression Objective: To investigate the human urinary kinin original enzyme (HUK) on cerebral ischemia reperfusion after ischemic side cortex nestin (nestin) expression and spatial learning and memory disorders. Methods: The suture method middle cerebral artery occlusion (MCAO) and reperfusion model HUK treatment group at 2 weeks after intraperitoneal injection of HUK, the first 15 days the rats rats using the Morris water maze device evaluation space learning and memory abilities, Nissl staining the ischemic side changes in the number of cortical neurons, the immunohistochemical staining ischemic side cortex nestin expression. Results: cerebral ischemia in rats navigation test and spatial probe test showed significant spatial cognitive dysfunction. Navigation trial, HUK, high-dose treatment of rats average escape latency ischemic group was significantly shorter (P lt; 0.05). Space exploration test the HUK, high-dose treatment group rat primary platform quadrant dwell time percentage, and through the original platform location are greater than the number of cerebral ischemia group (P lt; 0.05). Ischemic cortex Nissl staining showed that the HUK, high-dose treatment group ischemic cortex survival neurons significantly higher than the number of cerebral ischemia group (P lt; 0.05). Ischemic side cortex nestin immunohistochemical staining results show that the the HUK, high-dose treatment group rat the ischemic cortex nestin optical density value was significantly higher than the cerebral ischemia group (P lt; 0.05). Conclusion: HUK can significantly improve cerebral ischemia reperfusion spatial learning and memory, the mechanism may increase with HUK the ischemic cortex nestin expression to promote nerve regeneration.

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  4. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  5. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  6. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  7. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  8. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  9. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  10. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  11. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  12. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  13. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  14. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  15. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  16. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  17. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  18. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  19. The Signal Mechanism Underlying DON Induced Apoptosis in BHK-21 Cells,S856.9
  20. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  21. The Study of Mitochondrial Signal Mechanism Induced Apoptosis by Don in Human Colon Cancer Cells,R735.35

CLC: > Medicine, health > Neurology and psychiatry > Neurology > Cerebrovascular disease > Acute cerebrovascular disease ( stroke)
© 2012 www.DissertationTopic.Net  Mobile