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Objective: To investigate serum adiponectin (adiponectin, APN) level of 276 genes single nucleotide polymorphisms (single nucleotide polymorphism at position 276, the SNP 276) and Yanbian Yanbian Han, Korean type 2 diabetes ( type 2 diabetes-mellitus, T2DM) and carotid intima - media hypertrophy degree of correlation (intima-mediathickness, IMT). Objects and methods: randomly selected the Yanbian region normal group (normal glucose tolerant, NGT) 190 cases, of which the Han 100 cases, Korean 90 cases; T2DM group of 217 cases, including the Han 123 cases, Korean 94 cases. T2DM group was divided into four groups based on carotid IMT: ① T2DM Han carotid atherosclerosis group (IMT ≥ 1.0mm and / or plaque formation); ② of T2DM Han group carotid atherosclerosis (IMT lt; 1.0mm and no plaque); 3 of T2DM Korean carotid atherosclerosis group (IMT ≥ 1.0mm and / or plaque formation); ④ of T2DM Korean the carotid atherosclerosis Group (IMT lt; 1.0mm and no plaque); Han, Korean of carotid atherosclerosis Group and no neck atherosclerosis group is divided into different depending on the measured genotypes GG genotype, the GT genotype and TT genotype group. Samples of serum adiponectin levels were measured by enzyme-linked immunosorbent assay, color Doppler assessment of carotid IMT, were genotyped using Taqman-MGB probe method, fluorescent sequencing method for the determination of the gene sequence, and record observations related to clinical indicators. The obtained data were statistically analyzed using SPSS13.0 software. : 1.T2DM group Han and Korean the carotid atherosclerosis serum APN level below without carotid atherosclerosis group, a significant difference (P lt; 0.05); the carotid atherosclerosis Group age, duration and higher than without carotid atherosclerosis group , a statistically significant difference (P lt; 0.05); 2. the Yanbian region NGT group and T2DM group SNP 276 genotype distribution characteristics for the TT (46.8% V.S14.3%), TG (27.9% vs 30.2%), GG (25.3% VS 46.5%); allele frequencies of T / G (60.8% V.S44.7%, 39.2% vs. 55.3%), two groups of genotypes and allele frequency distribution of a statistically significant difference ( X2 = 52.75, P lt; 0.001; X2 = 22.73, P lt; 0.001). 3 the Yanbian region NGT group Chinese and Korean SNP 276 genotype distribution characteristics TT (35.0% vs. 35.6%), TG (38.0% vs 36.7.9%), GG (27.0% VS 27.7%); their allele The frequency of the T / G (54.0% vs 754.4%, 46.0% V.S45.6%), two groups of genotypes and allele frequencies distribution was no statistical difference (P gt; 0.05). 4 the Yanbian area T2DM group Han and Korean SNP 276 genotype distribution characteristics for the TT (17.9% vs. 11.7%), TG (36.6% V.S41.5%), GG (45.5% V.S46.8%); allele frequency T / G (36.3% vs. 32.49%, 63.8% VS 67.67%), two sets of genotype distribution and allele frequency was no statistical difference (P gt; 0.05). Carotid atherosclerosis group of of Yanbian Han T2DM with carotid atherosclerosis group SNP 276 genotype distribution characteristics for the TG / TT (48.2% V.S56.5%), GG (51.8% V.S43.5%), two group genotype distribution without statistical differences (P gt; 0.05). 6 Yanbian Korean T2DM in patients with carotid atherosclerosis group with carotid atherosclerosis group SNP 276 gene distribution characteristics for the TG / TT (50 .. 0V.S58.2%), GG (50.0% V.S42.0%), The genotype distributions of the two groups no significant difference (P gt; 0.05). 7. Both T2DM carotid atherosclerosis group and T2DM without carotid atherosclerosis Group, the GG genotype patients compared with the TT genotype patients, the former body mass index, carotid IMT and course of the disease are higher than the latter, but the GG genotype in patients with serum lipid adiponectin levels lower than in patients with the TT genotype (P lt; 0.05). 8.Logistic regression shows: and the Yanbian region associated with carotid atherosclerosis risk factors: age (B = 0.101, OR = 1.106, P = 0.000), duration (B = 0.065, OR = 1.375, P = 0.028), glycosylated hemoglobin (glycosylated hemoglobin, HbA1C) (B = 0.253, OR = 1.290, P = 0.013), APN (B = -0.94, OR = 0.390, P = 0.000). The Conclusion: 1.APN level the Yanbian area T2DM and carotid IMT correlation. The 2.S NP 276T / G with the Yanbian area T2DM relevance. 3.SNP 276T / G was no difference in the Yanbian area population of Han Chinese and Korean T2DM. 4.SNP 276T / G Yanbian area T2DM carotid IMT. 5.APN T2DM carotid atherosclerosis protection factor.
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