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Chapter Agrobacterium-mediated genetic transformation of Brachypodium establish Brachypodium (Brachypodium distachyon) because of its small plant life cycle is short, small and simple genome growth conditions become the new model plant rice outside. Brachypodium in host and pathogen interaction, cytogenetics, the mutant BAC library construction, easy to expand and analyze it with wheat relatives by EST organizational culture and genetic transformation technology, and to prove it as a model the value of the plant. The test to the diploid ABR 6 and Hexaploid ABR 102 Brachypodium immature embryos as explants to study medium carbohydrate 2,4-D genome ploidy, hormone, hygromycin the screening concentration agroinoculation concentration callus induction, differentiation, rooting and conversion efficiency. The results showed that the callus induction medium: LS 2,4-D 2.5 mg · L-1 maltose 30 g L-1 agar 6.5 g · L-1; callus subculture medium: LS 4-D 1.0 mg · L-1 BA 0.2 mg · L-1 maltose 30 g L-1 agar 6.5 g · L-1; callus differentiation medium: LS KT 0.2mg · L-1 CuSO4 0.6 mg · maltose 30 g · L-1, L-1 agar 6.5 g L-1; callus rooting medium: LS IAA 0.6 mg · L-1 maltose 20 g · L-1 agar 7.0 g · L-1. Embryogenic callus derived from immature embryos the LS medium induced rate of 65.47%. Callus in medium containing 30 g · L-1 maltose, callus rate up to 96.67% in the medium containing 0.2 mg · L-1 KT's the differentiation rate up to 71.25%. ABR as Agrobacterium transformation of materials, the the hpt selective agent screening concentration of 40 mg L-1, Agrobacterium broth OD600 0.6, is the most efficient concentration of Agrobacterium-mediated transformation, the transformation frequency of 5.0% on 12 The strains resistant plants PCR molecular biology analysis, seven bands amplified 845 bp (hpt) and 535 bp (mGFP5 *). Transgenic plants was observed under a fluorescence microscope, the green fluorescent protein expression, and further proof of the reliability of the transgenic plants. Were optimized the callus culture conditions, Agrobacterium-mediated genetic transformation system Brachypodium. Chapter II the wheat TaOZR gene cloning and analysis of wheat stripe rust caused by wheat stripe rust (Puccinia striiformis f. Sp. Tritici) is an important disease of wheat production on a serious impact on the yield and quality of wheat. Studies have shown that the most effective way to control wheat stripe rust is to cultivate disease-resistant varieties, and to reveal the molecular mechanism of the interaction between wheat and stripe rust is to cultivate the premise of the theory of disease-resistant varieties. Silico cloning and RT-PCR method, the test of wheat infected with stripe rust water 11 isolated a cDNA sequence coding OZR. Bioinformatics sequence analysis of the cDNA sequence and its encoded protein; using real-time quantitative PCR (qRT-PCR) analysis of the gene in wheat stripe rust interaction induced expression of exogenous hormones and abiotic stress situation, study its role in the defense response of stripe rust resistance and abiotic stress process. Silico cloning wheat OZR, named TaOZR. ORF of 240 bp, encoding 80 amino acids; theoretical molecular weight of 8.67 kD protein sequences, isoelectric point 9.34; signal peptide and transmembrane region, may be secreted proteins; similarity rice OZR up 76%; TaOZR Stripe Rust after induction in affinity interactions in the difference is not significant, and upregulated in affinity interaction induced expression; exogenous plant hormones salicylic acid, ethylene, abscisic acid, jasmonic acid induced TaOZR accumulation; cold, drought, The the high salt abiotic stress TaOZR expression was not significantly different. The trial was the first cloned into a stripe rust in wheat induced TaOZR, TaOZR possible through the synergy of salicylic acid, ethylene, abscisic acid and jasmonic acid signaling pathways involved in the early defense of wheat stripe rust.
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