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Biocompatibility of Pure Titanium Modified by Human Endothelial Cell-Derived Extracellular Matrix

Author: XueXiaoQing
Tutor: WangJin
School: Southwest Jiaotong University
Course: Biomedical Engineering
Keywords: Titanium Extracellular matrix Human umbilical vein endothelial cells Platelet adhesion Biocompatibility
CLC: R318.08
Type: Master's thesis
Year: 2010
Downloads: 87
Quote: 3
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Abstract

Using a single component of the extracellular matrix material surface modification of cardiovascular, endothelial improve the material in the body of the capacity is limited. Biomedical materials to build cell surface autocrine extracellular matrix (ECM), contain cell adhesion, growth, reproduction, promote the role of a variety of active factor, it has other biological methods can not match the advantages of the biomedical materials Surface Modification great potential value in the application. In this paper, acellular method titanium (Ti) polished group (P) and acid etching group (AT) cells from the surface of the sample build secretion of extracellular matrix in order to achieve biological material surface modification. Characterized by Fourier transform infrared spectroscopy (FTIR), photoelectron spectroscopy (XPS) and contact angle measurements for building ECM front surface of the material composition and physico-chemical properties of characterization and analysis; using atomic force microscopy (AFM), optical microscopy and scanning electron microscopy ( SEM) for surface morphology; built by immunofluorescence staining on the surface of the material before and after ECM type Ⅳ collagen, fibronectin (FN) and laminin (LN) for qualitative identification and analysis; also by in vitro human umbilical vein endothelial cells (HUVEC) culture experiments, cytoskeleton (actin) Immunofluorescence staining experiments and experimental evaluation of platelet adhesion to ECM built around titanium surface biocompatibility and blood compatibility. FTIR results showed that the two groups after building ECM Ti sample surface are characteristic groups of proteins appear amide Ⅰ and Ⅱ amide groups; XPS results showed that the two groups after building ECM Ti O and N elements of the sample surface binding energy changes; optical microscopy and SEM photos show the two groups after building ECM and Ti surface of porous mesh structure; AFM ECM is further confirmed build more smooth polished Ti surface topography; build ECM Ti sample surface after two pair of double-distilled water contact angle increases, hydrophilicity decreased, and two sets of samples the same trend; by immunofluorescence staining, build ECM after two Ti-rich sample surface FN, LN and type Ⅳ collagen; These results show that the ECM has been successfully built on two Ti sample surface. In vitro cultured human umbilical vein endothelial cells by light microscopy, SEM and Alamar Blue experimental results show that the two groups after building ECM Ti sample surface endothelial cell adhesion and proliferation activity than did not build the ECM sample surface, and showed significant differences, This shows that in the pure Ti surface build cells after autocrine ECM has good biocompatibility. Actin cytoskeleton immunofluorescence staining showed that the two groups after building ECM Ti sample surface actin cytoskeleton neat, compact and was completely spread state, while the cells on the surface of the sample build ECM cross-linking between filaments arranged messy, and cell surface roughness uneven. Again, the sample surface constructed of Ti ECM cells were significantly increased, indicating that ECM not only to promote endothelial cell growth, but also arranged in the cytoskeleton. Platelet adhesion in vitro experiments showed that the two groups to build cell Ti sample surface after both autocrine ECM can inhibit platelet adhesion and activation on its surface, thereby increasing the surface anti-clotting properties. The above test results show that the acellular titanium successfully by the sample surface in two cells in an autocrine constructed ECM. ECM on endothelial cell growth has a good role in promoting pure titanium can inhibit platelet adhesion and activation of the surface, thereby increasing its surface anticoagulant properties. Therefore, building on titanium surface cells autocrine ECM is to improve the surface biocompatibility effective biological modification method.

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CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > General issues > Biomaterial
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