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Only in everyday life and clinical application of hemostatic dressings mere hemostatic effect; biological dressings have been developed in recent years also applies only in a superficial wound clean, aims to promote wound healing the Battle trauma wounds, especially naval battle injury the lack of specificity. Our group developed a new type of composite chitosan, calcium alginate and polyvinyl alcohol (PVA) dressing to study set hemostasis, anti-inflammatory, waterproof, promote healing, no need to replace a short period of time advantage in one composite type dressings are available, there is an important role in reducing the seawater immersion wounded disability and death. Through animal studies the dressing with the above-mentioned functions and provide the basis for clinical application. The first part of the experimental study of the hemostatic properties of chitosan / alginate dressings [Objective: to study the new dressing: chitosan / alginate dressing (the project group self-developed, has applied for national patent) hemostatic properties. [Method]: Take four New Zealand rabbits were cut 5 1cm in diameter circular wound in the back on both sides of the symmetry, respectively, with quite a few of chitosan / alginate dressings and gelatin sponge hemostatic wound size, observation and wound sticky attached to the case, records bleeding time; stop the bleeding stopped, the chitosan / alginate dressings and gelatin sponge into the pre-preparation of good cyanide methemoglobin detection reagents carefully cleaned using colorimetric spectrophotometer at 540nm wavelength photometric Hb luminosity measured absorption values ??expressed blood. [Results]: chitosan / alginate dressings and wound adhesion is better, chitosan / alginate dressing group, the gelatin sponge group of bleeding time were 87.7 ± 19.1 seconds, 170.7 ± 22.6 seconds, Hb luminosity absorption values ??were 1.131 ± 0.44,1.733 ± 0.733, the statistical analysis, the two sets of data has a significant difference (P lt; 0.01), chitosan / alginate dressing group was significantly better than the gelatin sponge. [Conclusion]: chitosan / alginate dressings have good hemostatic properties. The second part of chitosan / alginate dressings seawater immersion wound healing experimental study [Objective] chitosan (chitosan, CTS) / alginate (alginate, ALG) the dressing on rats seawater soaked wounds. [Methods] 25 healthy SD rats, weighing 250 ~ 300 g, at the spine on both sides cut 1.8 cm diameter circular wounds, making the skin wound model, the experimental group on the left, the right side of the control group. Artificial seawater soak the wound in a pre-configured for 1 h, the experimental group using the CTS / ALG dressings and topical, control group, with sterile gauze and topical gross observe and record the time of wound healing; postoperative 3,5,7,10,12 d cut wounds including 2 cm × 2 cm skin specimens, HE staining and immunohistochemical Envision staining observed wounds histological changes and EGF, bFGF expression. [Results] The experimental group wound inflammatory response light scab shrink faster; the wound scab contraction slower control group. Experimental group wound completely healed (11.68 ± 0.57) d, and the control group (12.51 ± 0.54) d, and the difference was statistically significant (P lt; 0.05). The experimental group turn since the first 3 d after the beginning of the granulation tissue and cell infiltration, collagen tissue, wound shrinkage and epithelial preoperatively to 10 d, collagen tissue has been arranged in neat rows, epithelialization process basically completed, visible skin attachments appeared completely healed after 12 d; corresponding time points of the control group cell infiltration appears late in the granulation tissue ulceration epithelialization process late. Immunohistochemical observed in the experimental group were 3,5 d bFGF in vascular endothelial cells and stromal fibroblasts high expression, EGFR is highly expressed in vascular endothelial cells; 7,10,12 d postoperatively showed low expression; control group patients after the corresponding time points showed low expression and no expression. [Conclusion] CTS / ALG seawater soaked wounds promote healing, but the mechanism remains to be further in-depth study.
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