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The Correlation Analysis on Enterococcus Faecalis Biofilm and Clinical Manifestation in Retreatment Root Canal

Author: ZhengJianBo
Tutor: NiuWeiDong
School: Dalian Medical University
Course: Stomatology
Keywords: Enterococcus faecalis Endodontic Biofilm
CLC: R781.05
Type: Master's thesis
Year: 2010
Downloads: 156
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Abstract


Again purposes : screening and identification of Enterococcus faecalis in root canal treatment , detection of Enterococcus faecalis, the ability to form biofilms , to explore the relationship between the ability of biofilm formation and clinical manifestations . Methods: 135 of the 118 patients need root canal treatment teeth ( each teeth only take a root canal ) , record the teeth of clinical symptoms , signs and information . Disinfection original filling , sterile root canal file to remove root canal filling , with reference to the total length of the root canal of the X-ray image , sterile paper points enclosed root canal 60s samples of the bacteria in the root canal teeth removed sterile dental drill The selective medium screening and analyzer of automated microbial identification within the root canal Enterococcus faecalis . Faecalis Biofilm vitro model establishing the clinical samples in a 96 - well plate , using crystal violet staining and microplate detection in clinical samples isolated Enterococcus faecalis ability to form biofilms , and statistical analysis of its biofilm formation capacity the relationship between the clinical manifestations . Results: After the identification of selective media screening and automatic microbial analyzer , 135 Enterococcus faecalis root canal treatment detected 52 cases , the detection rate was 38.5% . Detection of root canal treatment Enterococcus faecalis biofilm formation ability , found in 36 (69.2 %) of the biofilm formation capacity , of which two (3.8%) with strong biofilm forming ability, 34 ( 65.4% ) with moderate biofilm forming ability. Statistical analysis showed that fistula treatment of root canal fecal enterococci biofilm formation correlation results were statistically significant (0.0 lt; P lt ; 0.05 ) and other clinical manifestations and Enterococcus faecalis biofilm formation was not statistically significance (P gt; 0.05). Conclusion : (1) the majority of clinical isolates of Enterococcus faecalis can the ability to form biofilms . Root canal treatment , no the fistula teeth separated Enterococcus faecalis biofilm formation ability in Enterococcus faecalis biofilm formation ability the fistula teeth separated .

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CLC: > Medicine, health > Oral Sciences > Oral medicine > Therapy
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