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Objective To investigate the ocular applications peroxidase enzyme proliferator activated receptor ( peroxisome proliferator -activated receptor the gamma , PPAR -y ) agonist pioglitazone (Pioglitazone) on rats corneal alkali burn nuclear transcription factor -kappaB ( nuclear factor -kappa B, NF-κB) and tumor necrosis factor -alpha ( tumor necrosis factor- alpha , TNF -a ) expression . Methods 48 healthy SPF SD rat corneal alkali burn randomized groups of 12 mice (12 eyes ) , Ⅰ group for burn treatment group (Ⅰ A low- dose group ; Ⅰ B high-dose group ) , respectively subconjunctival injection 0.2 g / L, 0.8g / L pioglitazone pioglitazone 0.1ml, day 1. Ⅱ group for the treatment of the control group , subconjunctival injection of dexamethasone , 0.5mg 0.1ml, 1 time per day . Ⅲ group was the control group , the subconjunctival injection of saline 0.1 ml of 1 time per day . All rats right eye for the experimental eye , the left eye as a negative control . Continuous medication for two weeks . Dynamic detection alkali burn one day , 4 days , 7 days , 14 days of corneal neovascularization (corneal neovascularization, CNV) development , as well as observation of PPAR-γ , NF -κB and of TNF- a in each time segment expression. PPAR-γ since burn one day begin to express , with the development of CNV occurrence of PPAR-γ , NF - κB , TNF - a expression of an upward trend . CNV incidence Ⅰ B group compared to the group Ⅱ , Ⅲ group was significantly delayed growth inhibition ; I Group A comparison between the two groups with Group II , the difference was not statistically significance ( P gt ; 0.05) . Ⅰ B group 4, 7, 14 days CNV growth area , respectively : ( 5.42 ± 0.25 ) mm2 ( 8.14 ± 0.25 ) mm2 ( 9.67 ± 0.42 ) mm2 compared between groups differences were statistically significant (both for P lt; 0.05 ) ; 7 days after alkali burn Ⅰ B group the expression of PPAR-y than group Ⅱ , Ⅲ group was significantly increased , NF-κB, TNF-a expression in varying degrees of decline , the differences were statistically significant . (P lt; 0.05) Conclusion of PPAR- y agonists pioglitazone pioglitazone inhibit rat CNV generation , to reduce the inflammatory response and dose levels . Its mechanism may be related to the activation of PPAR- y , early in the inflammatory effectively prevent NF-κB activation and reduce the expression of TNF-a .
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