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The Study of CD133+ Cells and Its Relationgship with Angiogenesis and VEGF in Colorectal Cancer

Author: ShangLiNa
Tutor: LiMin
School: Lanzhou University
Course: Pathology and Pathophysiology
Keywords: CD133 Colorectal cancer stem cells VEGF Microvessel density (MVD) Endothelial cells
CLC: R735.3
Type: Master's thesis
Year: 2010
Downloads: 124
Quote: 0
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Abstract


Objective: ( 1 ) to study the relationship between CD133, vascular endothelial growth factor expression and clinical pathological features in 94 cases of colorectal cancer tissue samples to explore the correlation between CD133 expression and tumor microvessel density (MVD), VEGF expression sex . (2) separation from colon cancer cell line SW480 CD133 cells and CD133 cells express VEGF detection of endothelial cells . Methods : ( 1) the two-step method and the tissue microarray technique using SP immunohistochemical detection of CD133, VEGF, CD31 expression in 94 cases of colorectal cancer specimens . (2) the use of immunomagnetic bead separation technology and serum-free suspension culture Enrichment of CD133 cells . SW480 cells or beads sorting CD133 cells were cultured under culture conditions using immunohistochemical method to detect CD133 and VEGF expression in six groups of cells after various incubation times . Results : ( 1 ) colorectal cancer CD133 expression and the degree of tumor differentiation and lymph node metastasis (P lt; 0.05). (2) a negative correlation between colorectal cancer tissues of CD133 expression and VEGF expression and MVD . (3) colorectal cancer tissue microvascular density press CD133-/VEGF- , → CD133 / VEGF - → of CD133 / VEGF → CD133-/VEGF , the order is gradually increased . (4) in serum-containing medium or a serum - free medium , SW480 cells were co-cultured with endothelial cells the CD133 cell ratio , and do not add endothelial cells than those who are increased ( P LT ; 0.05 ) . ( 5) in serum-free medium , SW480 cells and CD133 cells add the ratio of VEGF in endothelial cells induced the expression of CD133 cells a significant increase ( P lt ; 0.01 ) ( 6 ) after various incubation times , six groups under different culture conditions CD133 positive cells and VEGF expression ratio between cells is negatively correlated . Conclusion: ( 1) the endothelial cells in the colon may promote colon cancer stem cell self-renewal . ( 2 ) endothelial cells may promote the occurrence of vascular expression of VEGF -induced colon cancer stem cells . (3) CD133 cells may be the through autocrine or next to secrete VEGF way to promote angiogenesis , the microvascular density increased promotion of CD133 cell differentiation and maturation .

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Intestinal neoplasms
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