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Research on Preparation and Anti-hepatoma of Docetaxel Carried Carbon Containing Iron Nanoparticles

Author: LiCaiKun
Tutor: CaiHongPei
School: Second Military Medical University
Course: Internal Medicine
Keywords: Hepatoma cells Carbon-coated iron nanoparticles Docetaxel Apoptosis
CLC: R735.7
Type: Master's thesis
Year: 2010
Downloads: 90
Quote: 0
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Abstract


Liver cancer is a common malignancy, a highly malignant biological behavior and poor clinical prognosis. The inhibitory effect of conventional chemotherapy drugs HCC is limited, and the chemotherapy drug cytotoxicity, specificity, and likely to cause damage to the liver and other organ systems in the process of destruction of liver cancer cells. Therefore, the study of primary liver cancer cytotoxic chemotherapy drugs are mainly focused on the improvement of the route of administration and method of administration. In recent years, clinical anticancer drug emerging docetaxel (Docetaxel, DTX) in paclitaxel based on the synthesis of new anticancer drugs, was considered one of the most effective chemotherapeutic single drug, but if followed conventional method of administration and the route of administration of the tumor outside the cell damage is inevitable. Recent molecular targeted technology development to bring new opportunities for the clinical treatment of liver cancer, the development of nanotechnology and nanomaterials equipped with chemotherapy drugs continue to be applied in cancer research, and made a good therapeutic effect. This topic on carbon-coated iron nanoparticles (Carbon-containing iron nanoparticles, CCINs) equipped with DTX study and observe its cytotoxic effect on liver cancer cells, new ways to explore the treatment of liver cancer and DTX application results provide preliminary experimental evidence. Chapter carbon-coated iron nanoparticles carrying docetaxel prepared Objective To investigate the effect of CCINs DTX equipped, and to evaluate the physical and chemical properties, and to explore carbon-coated iron nanoparticles - preparation of docetaxel (CCINs-DTX) process. Method using phacoemulsification - the solvent evaporation equipped with DTX synthesis technology CCINs and colorimetric evaluation of their drug loading, encapsulation efficiency, release performance, and study its magnetic laser particle size instrument investigate its stability. Its drug loading of 274 mg / g, and the encapsulation efficiency was 82.74%, to quickly reach an effective concentration, and the ability to maintain a stable high drug concentrations, CCINs-DTX stable results after phacoemulsification - solvent synthesis CCINs-DTX detection Detection of its three months diameter no significant changes, with good stability, and magnetic detection shows that it has a magnetic response. The study concluded by phacoemulsification - solvent synthesis techniques CCINs-DTX formulations having good drug loading, encapsulation efficiency, release performance and stability, and having good magnetic response. Chapter II CCINs-DTX on cultured liver cancer cell growth and apoptosis of research study Objective To observe CCINs-DTX on cultured liver cancer cell growth and apoptosis, and to explore the mechanism of its anti-tumor. Research methods in vitro cultured hepatoma cells were randomly divided into four groups: drug group (DTX) the microsomal drug group (CCINs-DTX), blank group (NS) and blank microspheres group (CCINs). The DTX intervention drug group, particulate drug group CCINs-DTX intervention control group with saline intervention, blank micro the the ball group using CCINs intervention. Groups of cell growth inhibition rate using colorimetric assay, flow cytometry apoptotic index of cells in each group, comparing groups of cell growth inhibition rate and apoptotic index. Findings lt; 80mg / L drug concentration, 24h, 48h and 72h detection showed that drug group with the drug particles group cell inhibition rate without significantly with differences; gt; 80mg / L drug concentration, 24h, 48h and 72h detection showed that group of drug particles cell growth inhibition rate was higher; particles group compared with the control group, did not show inhibition of cell growth; compared with the control group and particulate group, drug group and drug particles group cell apoptosis rate increased; same drug group compared with the drug particles apoptosis rate increased; the particulate group in comparison with the control group, no significant difference in the rate of apoptosis. Conclusions CCINs-DTX hepatoma cell growth in vitro was significantly inhibited, play the inhibition of liver cancer cells by promoting apoptosis of hepatoma cells.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
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