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The Correlation Between Mitogen-activated Protein Kinase Signal Transduction Pathway and Human Sperm Motility

Author: DingYuQin
Tutor: JiangHong
School: Anhui Medical University,
Course: Obstetrics and Gynaecology
Keywords: Sperm Activity Mitogen-activated protein kinase (MAPK) Extracellular signal-regulated kinase (ERK) P38 mitogen-activated protein kinase (P38 MAPK) PD98059 SB203580 Protein kinase C (PKC) Immunoblotting Flow cytometry (FCM) Phosphorylation Protein Signal Transduction
CLC: R698.2
Type: Master's thesis
Year: 2010
Downloads: 103
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Abstract


The first part of the extracellular signal-regulated kinase (ERK) and P38 MAPK expression and human sperm motility correlation Objective: To compare the extracellular signal-regulated kinase (ERK), P38 mitogen-activated protein kinase (P38MAPK) phosphorylation and protein expression levels in normal sperm and sperm asthenospermia differences in patients to investigate the expression levels of ERK and P38MAPK sperm motility and density correlation. Methods: The study subjects from the People's Liberation Army 105 Hospital infertility clinic, patients conventional abstinence from 3 to 7 days, take the semen by masturbation in a sterile cup, liquefied underwent routine examination, collecting normal semen (sperm concentration ≥ 20 × 106 / ml , a ≥ 25% or ab ≥ 50%) and a weak sperm in semen (sperm concentration ≥ 20 × 106 / ml, ab ≤ 40%) of the 20 parts of the sperm washing total protein was extracted using immunoblotting (Western Blotting) were detected ERK, P38 MAPK phosphorylation and protein expression levels. Results: ERK and P38MAPK normal sperm and sperm asthenozoospermia patients were expressed, ERK protein expression levels and phosphorylation levels P38MAPK in asthenospermia patient group was significantly higher (P lt; 0.05), and ERK phosphorylation between the two groups the difference was not statistically significant level (P gt; 0.05); groups were associated with the sperm density ERK, P38MAPK protein expression levels were not significantly correlated (r1 = 0.086, r2 = 0.121, P are gt; 0.05). CONCLUSION: Human sperm ERK activity decreased and P38 MAPK activity increased low sperm motility may be one reason; but ERK and P38 MAPK protein levels may be associated with spermatogenesis irrelevant. The second part of the mitogen-activated protein kinase (MAPK) inhibitors on human sperm motility and its mechanism Objective: To investigate the extracellular signal-regulated kinase (ERK) inhibitor PD98059 and P38 MAPK inhibitor SB203580 on sperm motility and its mechanism. Methods: The study subjects from the People's Liberation Army 105 Hospital infertility clinic, patients conventional abstinence from 3 to 7 days, take the semen by masturbation in a sterile cup, liquefied underwent routine examination, normal semen were collected (sperm concentration ≥ 20 × 106 / ml, a ≥ 25% or ab ≥ 50%) and a weak sperm in semen (sperm concentration ≥ 20 × 106 / ml, ab ≤ 40%) of 15 parts, washing, adjust the sperm density, respectively, in normal sperm and weak sperm sperm with different concentrations of PD98059 and SB203580, testing different time changes in sperm motility, and using immunoblotting (Western Blotting) inhibitors were detected before and after intervention phosphorylation levels of ERK and P38MAPK changes. Results: 80μmol/LPD98059 can significantly inhibit the normal sperm motility, 40min to effect the most significant; while 60μmol/LSB203580 can be significantly increased in patients with weak sperm motility, in order to effect the most significant at 10min; two inhibitors can reduce ERK and P38MAPK phosphorylation levels. Conclusion: PD98059 and SB203580, respectively, and by blocking ERK signal transduction pathway P38MAPK affect sperm motility, ERK and P38MAPK regulate sperm motility is an important signal transduction pathways. The third part of the protein kinase C (PKC) on human sperm motility and its mechanism Objective: To investigate human sperm protein kinase C (PKC) expression levels on sperm motility and its mechanism. Methods: The study subjects from the People's Liberation Army 105 Hospital infertility clinic, patients conventional abstinence from 3 to 7 days, take the semen by masturbation in a sterile cup, liquefied underwent routine examination of semen were collected 30 cases of normal sperm (sperm density ≥ 20 × 106 / ml, a ≥ 25% or ab ≥ 50%) and 20 parts of asthenospermia semen (sperm concentration ≥ 20 × 106 / ml, ab ≤ 40%) as a normal control group and weak azoospermia group. Were washed, followed by flow cytometry (FCM) were detected sperm PKCβ Ⅰ content; and normal sperm by density gradient centrifugation, were added PKC activator PMA and inhibitors GF109203X, using immunoblotting (Western Blotting) Methods After testing the role of PMA and GF109203X ERK phosphorylation and protein expression level changes. Results: weak sperm sperm PKCβ Ⅰ group was significantly lower than the normal group; adding sperm after PKC activator PMA ERK phosphorylation levels were significantly increased by adding after phosphorylation of ERK inhibitor GF109203X significantly reduced. Conclusion: the reasons for low sperm motility and the decreased content of PKC, PKC through MAPK signal transduction pathways involved in regulating sperm motility.

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CLC: > Medicine, health > Surgery > Urology ( urinary and reproductive system diseases) > Men 's sexual dysfunction > Male infertility
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