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Effects of Seromuscular Anastomosis on Anastomotic Healing of Colon in Rats

Author: GaoXiangYu
Tutor: HuXiang
School: Dalian Medical University
Course: Surgery
Keywords: The urothelium Hydroxyproline Burst pressure Type I collagen CD146
CLC: R656.9
Type: Master's thesis
Year: 2010
Downloads: 23
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Abstract

Objective: The purpose of this experiment is to explore the possibility of joints and submucosa of the colon lined with consistent, with the traditional manual method of matching - a layer anastomosis, postoperative colonic anastomotic healing quality. Methods: healthy and clean 48 Wistar rats, female, weighing 240-270g. Were randomly divided into two groups: group A layer anastomosis group, B group the urothelium anastomosis group, n = 24, individual cages. Right colon amputation stump anastomosis. 3, 7 and 14 days, respectively, after each group were randomly selected eight rats were sacrificed, cut colonic anastomotic surrounding tissue. 1 Determination of the index of abdominal adhesions. 2 - Determination of colonic anastomotic bursting pressure (ABP). Determination of colonic anastomotic tissue hydroxyproline (HP) content. 4 HE staining, routine pathological examination. Line CD146 immunohistochemical staining. Determination of MVD count. Line type I collagen immunohistochemical staining. 8.RT-PCR assay relative expression levels CD146mRNA. 9.Western blot assay relative expression levels of CD146 protein. 10 Measurement data were expressed as mean ± standard deviation (xs), using one-way ANOVA, P <0.05 was considered statistically significant. Results: 1. Intraperitoneal adhesion Index: tests showed anastomosis group of the urothelium was significantly lower than in 3, 7 and 14 days after abdominal adhesions index layer anastomosis group was statistically significant (P <0.05). Anastomotic bursting pressure (ABP): 3 days after the urothelium anastomosis group than layer anastomosis group is slightly lower, but no significant difference (P> 0.05). 7 days after the urothelium anastomosis group ABP rise above the layer anastomosis group, but not statistically significant (P> 0.05). After 14 days, the two groups ABP close (P> 0.05). 3 content of hydroxyproline (HP): The overall postoperative colonic anastomotic tissue hydroxyproline content over time upward trend. To three days after the two groups hydroxyproline content (P> 0.05). After 7 days, 14 days lined with anastomosis group hydroxyproline content increased significantly, higher than that of the layer anastomosis group (P <0.05). HE staining: pathological observation showed an increase in neutrophils 3 days after layer anastomosis group than the urothelium anastomosis group, a large number of necrotic cells disordered arrangement. 7 days after the decrease in neutrophils, monocytes, lymphocytes increased. The urothelium anastomosis group granulation tissue formation. Contains a large number of fibroblasts and neovascularization, neovascular vertical wounds growth of, compared with a layer anastomosis group. 14 days after the urothelium anastomosis healing was significantly better than a layer of anastomosis group, the cells arranged in neat rows. 5.CD146 immunohistochemical staining: pathological observations indicate that the urothelium anastomosis group cytoplasmic CD146 expression of vascular endothelial cell the 7-Tiancheng strong positive positive 3 days after injury, injury, and 14 days after injury positive weakened; layer anastomosis group vascular endothelial cell cytoplasm the CD146 expression Tiancheng positive 3 days after injury partially positive, partially weakly positive after injury after injury, 14 Tiancheng positive. Value 6.MVD: the urothelium anastomosis MVD was significantly increased 3 days after the 7th day reached a high level, then reduce to a lower level, 14 days after. Layer anastomosis group 3 days, 14 days MVD value slightly lower than the urothelium anastomosis group, but no significant difference (P> 0.05). 7 days after the value of MVD was significantly lower than the urothelium anastomosis group (P <0.05), type I collagen area: after 3 days between the two groups was no significant difference (P> 0.05). After 7 days, 14 days lined with type I collagen area of ??the anastomosis group than the layer anastomosis group have increased significantly, with significant difference (P lt; 0.05). The 8.CD146mRNA expression: After 3 days, the relative expression levels of the two groups no significant difference (P> 0.05). After 7 days, 14 days colon lined with anastomosis group relative expression levels increased significantly, with a layer of anastomosis group difference (P <0.05). 9.CD146 relative expression levels of the protein: After 3 days, the relative expression levels of the two groups no significant difference (P> 0.05). 7 days after the colon lined with anastomosis group CD146 protein expression was significantly increased, a larger difference (P <0.05) after 14 days in both groups CD146 relative expression levels are significantly lower layer anastomosis group, but no significant difference between the two groups (P> 0.05). Conclusions: 1. Colon lined with anastomosis due to abdominal adhesions than light layer anastomosis. Anastomosis of the urothelium and a layer anastomosis as safe and reliable. 3. Lined with anastomosis group hydroxyproline content than the layer anastomosis group increased. Anastomosis group of the urothelium of type I collagen increased the size of a layer of anastomosis group. Lined with vascular anastomosis group number than layer anastomosis group increased. Gastrointestinal tract lined with anastomosis is a safe, reliable and consistent than the other manual easier way.

Effects of Seromuscular Anastomosis on Anastomotic Healing of Colon in Rats

Abstract

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