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Anti-metastasis of Resveratrol and Luteolin in Mice with Breast Cancer

Author: ZhangShuo
Tutor: DuGangJun
School: Henan University
Course: Pharmacology
Keywords: Resveratrol Luteolin 4T1 breast cancer Lymphocytes Antimetastatic
CLC: R737.9
Type: Master's thesis
Year: 2010
Downloads: 76
Quote: 0
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Abstract


Breast cancer is a malignant tumor incidence increased gradually in recent years, so it's highly metastatic patient survival was significantly reduced. Metastasis involves a complex, multi-stage immune escape. Malignant tumor incidence of severe suppression and destruction of the patient's immune function, radiotherapy, chemotherapy and other treatments are also caused by the different aspects and different levels of immune function, provided the conditions for diffusion transfer of tumor cells. Flavonoids resveratrol and luteolin has anti-inflammatory, anti-oxidation, scavenging free radicals, anti-cancer effects. This topic emphasis on both the anti-breast cancer metastasis. MTT assay of resveratrol and luteolin in vitro 4T1 breast cancer cells and spleen T, B lymphocytes; spontaneous lung metastasis model, artificial lung metastasis model and in situ in breast cancer metastasis and recurrence after resection of the experimental evaluation of Chenopodium quinoa Lo alcohol and luteolin in vivo anti-breast cancer metastasis; hydrogen peroxide damage normal mouse spleen lymphocytes, single cell gel electrophoresis experiments and 4T1 breast accompanied by an immune response to explore resveratrol and luteolin anti-breast cancer metastasis mechanism. Vitro antimetastatic results show that: both hypoxic and normoxic environment, resveratrol and luteolin in drug concentration 100μmol / L or less can significantly inhibit the proliferation of 4T1 breast cancer cells induced by ConA; drug concentration 20μmol / L proliferation of T lymphocytes have a significant role in promoting; on LPS-induced B lymphocyte proliferation resveratrol inhibited luteolin to promote the role of performance in normoxia, hypoxia showed inhibition. The in vivo the antimetastatic results show: 4T1 breast cancer spontaneous lung metastasis and experimental lung metastasis, resveratrol and luteolin could significantly reduce the number of metastatic lung nodules. Luteolin can inhibit the growth of breast cancer in situ tumors, end of treatment, prevention and treatment groups tumor inhibition rate of 41% and 44%, respectively. Two drugs metastasis and recurrence of breast cancer surgery had no significant effect. The mechanism results: resveratrol and luteolin drug concentration 20μmol / L can inhibit lymphocyte DNA chain hydrogen peroxide caused damage, and resveratrol 1μmol / L luteolin 2μmol, / L treatment lymphocytes the trailing length and normal control group basically no difference. Administered two drugs tumor showed significant anti-metastatic effect, but no the accompanied tumor immune response was enhanced role. In summary, resveratrol and luteolin has significant anti-breast cancer metastasis mechanism to kill tumor cells directly, enhancing immune function, protecting the nucleus of lymphocytes, inhibition of DNA damage in the pathological environment may There is a more complex immunomodulatory mechanism.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Breast tumor
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