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Dynamic Expression of IL-1β and IL-1β Type Ⅰ Receptor in the Rats of Bleomycin-induced Pulmonary Fibrosis

Author: WangXiaoHui
Tutor: LiuXueJun
School: Shanxi Medical
Course: The elderly respiratory disease to learn
Keywords: Pulmonary fibrosis IL-1β Type I receptor
CLC: R563.9
Type: Master's thesis
Year: 2010
Downloads: 88
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Abstract


Background: Pulmonary fibrosis is a common clinical pathological changes, a common outcome of a variety of unexplained interstitial lung disease, and often leads to pulmonary dysfunction and respiratory failure, a growing trend in recent years, it occurred more. Its mechanism is unclear, it still lacks an effective treatment for pulmonary fibrosis, the mortality rate is high, the prognosis is poor, it is to strengthen the study of the pathogenesis of pulmonary fibrosis, and based on the development of new therapeutic strategies to become very urgent practical needs. Currently, most scholars at home and abroad that played a very important role in the inflammatory cells release cytokines in the pathogenesis of pulmonary fibrosis. IL-1β is strongest in vivo role of pro-inflammatory cytokines, one of which is widely involved in the occurrence of the disease and development of a variety of body. Research reports, can be detected in bronchoalveolar lavage fluid in a rat model of pulmonary fibrosis and idiopathic pulmonary fibrosis patients, higher levels of IL-1β, IL-1β, however, in its main receptors The pathogenesis of pulmonary fibrosis, specifically how to dynamically change as yet unknown. This experiment is to study the establishment of an animal model of pulmonary fibrosis dynamic variation of IL-1β and its type I receptor in the development and progression of pulmonary fibrosis, which provide a new theory to further explore the mechanism and clinical treatment of pulmonary fibrosis basis. Objective: 1. Outlook Chabo Lai adriamycin-induced pulmonary fibrosis lung tissue in pathological changes. Determination of pulmonary fibrosis in rat lung tissue hydroxyproline content. Determination of pulmonary fibrosis in rat lung tissue, serum and bronchoalveolar lavage fluid dynamic expression of IL-1β and its type I receptor. Methods: 90 Wistar rats were randomly divided into normal group (n = 30), model group (n = 30) and methylprednisolone dragon group (n = 30), model group and the methylprednisolone group by intratracheal injection Rubo Lai mildew the hormone preparation pulmonary fibrosis disease model, normal group injected with saline. Modeling the same day, the methylprednisolone group treated by intraperitoneal injection of methylprednisolone, model group and the normal group was given saline, after once daily. Were 1,3,7,14,28 modeling rats (n = 6 / group) were sacrificed and specimens from lung tissue, HE staining, observed their pathological changes, alkaline hydrolysis was determined by hydroxyl proline concentration immunohistochemistry method for the determination of the content of the lung tissue IL-1β and its type I receptor; bronchoalveolar lavage fluid and serum, ELISA was used to detect the content of IL-1β and type I receptor . Results: 1. Pathological model group rats significantly widened alveolar septa, more fibroblast proliferation and infiltration of inflammatory cells, the late lung tissue consolidation; the methylprednisolone group phases pathological changes compared with the model group was significantly reduced. Hydroxyproline content of the model group and the methylprednisolone group with manufacturing die time increased gradually to 28 Telsda peak, and the model group was significantly higher than the normal group (P lt; 0.01), methylprednisolone The dragon group was significantly lower than the model group (P lt; 0.01). Immunohistochemistry results show: IL-1β and its type I receptor expression in the lung tissue of the model group scoring has been reduced in the first 7 days, 14 days, 28 days to express integrator with a regular set of basic equal; methylprednisolone group expressed 3,7,14 day scoring compared with the model group. 4.ELISA results show that: the model group reached a peak at day 7 in bronchoalveolar lavage fluid and serum IL-1β content at 14 days was significantly lower, but still higher than the normal group (P lt; 0.01), 28 days basic back to normal levels, the expression pattern of the type I receptor and IL-1β is basically the same. The methylprednisolone group IL-1β and its type I receptor expression levels at 3, 7, and 14 days lower than the model group (P lt; 0.01). Conclusion: IL-1β and its type I receptor was significantly higher in pulmonary fibrosis alveolitis stage, and a significant decrease in pulmonary fibrosis stage, showed an early and short-term peak expression in pulmonary fibrosis process, suggesting that it may play an important role early in the pulmonary fibrosis.

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