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Climacteric syndrome is a series of physical and psychological syndromes, which is caused by ovarian failure and endocrine disorder of women before and after menopause. Clinical manifestations are hectic fever with sweating, fatigue, listlessness, irritability, dizziness, headache, tinnitus, palpitation, insomnia, amnesia, lower back pain, burning sensation of hands and feet, menstrual disorder and so on. In recent years, due to aging of the population trends, the increasing social pressure and many other factors, the incidence rate of climacteric syndrome tends to increase.The application of hormone replacement therapy has a history of several decades in modern medicine, and today it is still the first choice for climacteric syndrome. However, in recent years, there are more and more reports on its various side effects. Therefore, to find safe and effective treatment gradually attracts people’s attention and becomes the research hotspot. Acupuncture is a kind of effective therapy for climacteric syndrome with less adverse reactions, and has very good prospects. But the selection of acupoints in clinical is primarily based on subjective judgments of doctors. The application of acupoints is complex, variable, and is lack of objective criteria and evidence.In this experiment, we have observed the influences of the levels of E2、FSH、LH and GnRH by EA at Taixi(KI 3), Sanyinjiao(SP 6), Guanyuan(RN 4) points in ovariectomized rats. The purpose of this study was to evaluate the regulatory effects of EA on HPO axis, investigate the specificity of acupoints and provide experimental evidences for acupoints selection and application in clinical practice.Main result are as following:1 In comparison with the normal group, E2 level in serum of the model group was significantly lower (p<0.01), the levels of FSH、LH and GnRH increased obviously(p<0.01; p<0.01; p<0.01).2 Compared with the model group, E2 level increased remarkably in each EA group (p<0.05; p<0.05; p<0.05); FSH、LH、GnRH levels decreased markedly (p<0.05; p<0.05; p<0.05).3 Comparison between the three groups, the serum E2 content was not significantly different (p>0.05); there were no obvious differences in the FSH、LH levels between EA Sanyinjiao group and EA Guanyuan group(p>0.05), FSH levels in both groups were remarkably lower than EA Taixi group(p<0.05), LH level in EA Guanyuan group was lower than EA Taixi group greatly(p<0.05); there was no apparently difference between EA Sanyinjiao group and EA Guanyuan group in GnRH content (p>0.05), but in both groups it was notably lower than EA Taixi group (p<0.05).Conclusions:1 EA could increase low estrogen level, inhibit elevated gonadotropin levels in ovariectomized rats and has benign regulation to disorganized HPO axis.2 The regulatory effect on the HPO axis of different acupoints is not identical. The role of acupoints has a certain specificity. Sanyinjiao and Guanyuan have the similar effect, which is obviously stronger than Taixi.3 The origination and development of climacteric syndrome involves in multiple organs, such as the liver, spleen and kidney, and is closely related with the Chong and Ren Meridians. The characteristics of Sanyinjiao, Guanyuan, and the results of this study can suggest that multiple organs, meridians, both innate and acquired fuctions should be considered all together, and in clinical acupoints election we should pay more attention to crossing acupoints for treatment of this disease.
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