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Parkinson's disease (Parkinson'sdisease, PD) is a slowly progressive disease of the central nervous degeneration. The main clinical manifestations of limb tremor, rigidity, bradykinesia and so on. The pathogenesis of PD and dopaminergic (dopamine, DA) neurons after injury, striatal dopamine and its metabolites decreased and so on. Further studies showed that the pathogenesis of PD and brain mitochondrial complex enzyme activity decreased and mitochondrial structure disorders. Current clinical treatment of PD no ideal way to acupuncture PD clinically significant results achieved gradually being recognized and utilized. But in exploring the mechanism of acupuncture treatment of PD is also not deep enough. Therefore, this experiment in establishing PD model, based on the proposed treatment of PD Effect of Acupuncture on the mechanism to do further research, applied behavioral science, HPLC, immunohistochemistry, biochemistry, electron microscopy and other techniques to observe acupuncture Du point decrease dopamine neuron loss and damage as well as regulating the role of DA and DOPAC, observation of the mitochondrial respiratory chain enzyme complex effects, from the perspective of improving mitochondrial function to explore acupuncture regulating governor Zhichan treatment of PD mechanisms for Acupuncture provide experimental evidence for treating PD. The experiment was divided into two parts: (a) by observing acupuncture Du \hydroxyphenylacetic acid (dihydroxyphenylacetic acid, DOPAC) study the effect of Acupuncture on PD protective effect of dopamine neurons; (2) observe acupuncture Du \respiratory chain enzyme complexes Ⅰ ~ Ⅳ activity, investigate Effect of Acupuncture protection mechanisms of brain mitochondrial function. Animal model and grouping: selection of C57BL / 6 mice with 0.3% MPTP (saline preparation) by intraperitoneal injection for 5 consecutive days, the establishment of animal models of PD. Animals were randomly divided into five groups: normal group, model group, acupuncture group, Madopar, acupuncture combined with Madopar group (referred to pin America group) survival of animals were 14d, 28d and 56d. The main results are as follows: 1, acupuncture, \Modeling 14d, model pole-climbing mice, the average time was significantly prolonged (p <0.05), compared with model group, acupuncture, needles America climbing pole time of mice had decreased (p <0.05), while the United States dopa mice climbing pole no longer than the model group was significantly shorter; 28d, three pole climbing treated mice compared with model group, shorten the time in which the needle America used mice climbing pole shortest time; 56 days, each group The average time of mice climbing pole was no significant difference. 2, acupuncture, \million more than the number of model group; 28d, model group 28 days the number of neurons in mice was significantly reduced compared with the normal group (P <0.05), the three treatment groups also decreased compared with the normal group (P <0.05), the number of groups of neurons needle America compared with Madopar group increased significantly (P <0.05); 56d, model group, the number of neurons was significantly less than the normal group (P <0.05), and needle acupuncture group were significantly more than the U.S. model group (P <0.05). 3, acupuncture, \three treatment groups was slightly higher than the model group, but not statistically significant; 28d, forebrain DA model mice were significantly lower than the normal group (p <0.05), the three treatment groups with no significant difference between the model group, the needle thorn Madopar group forebrain DOPAC content compared with the model group increased significantly (P <0.05). 4, acupuncture, \lysosomes and other changes; 14d, three treatment groups compared with the model group, to reduce the nuclear membrane invagination; 28d, acupuncture group and the group saw a small needle America mitochondria disappear, nuclear structure is clear, no significant retraction; 56d acupuncture group neuron mitochondria and some have disappeared, but lighter than Madopar groups, groups of neurons Madopar normal membrane structure is also visible retraction, visible cytoplasm mitochondrial cristae disappeared, but the number was compared with the model group small group of membrane structure Needle clear, no significant retraction, individual mitochondria cristae blurred or disappeared. 5, acupuncture, \; Madopar group at 14 days and 56 days of activity was also lower than the normal group. The acupuncture group and needle America activity in brain mitochondria no significant decrease (p <0.05). (2) on the impact of mitochondrial complex Ⅱ activity in 56 days in model group was significantly lower (P <0.05), the acupuncture group at 14 days and 56 days can improve brain activity of mitochondrial complex Ⅱ (P <0.05), the other two a treatment group and the normal group and model group than no significant difference (P> 0.05). (3) on the impact of mitochondrial complex Ⅲ 14d, 28d, no significant difference between the groups; 56 days, compared with the normal group, model group was significantly decreased (p <0.05), compared with model group, acupuncture group and needle America group inhibit Complex Ⅲ decrease (p <0.05). And Madopar group was significantly lower than the acupuncture group (p <0.05). (4) on mitochondrial complex Ⅳ impact 14d, no significant difference between the groups; 28d, model group activity was significantly decreased (P <0.05), the acupuncture group, acupuncture group than in the United States increased activity of the model group (P <0.05 ); 56 days, the model group activity was significantly decreased (P <0.05), Madopar, acupuncture group activity in the United States compared with the model group increased (P <0.05). Conclusion: These results suggest that acupuncture or acupuncture combined Madopar treatment to some extent to achieve regulating governor Zhichan, synergistic role attenuated, and its mechanism may reduce dopaminergic neuronal damage and reduce the loss of neurons , dopamine metabolism and improve the activity of mitochondrial enzymes. It is rich in acupuncture regulating governor Zhichan scientific connotation for clinical application of acupuncture treatment of Parkinson's disease Synergy and Attenuation modern scientific basis.
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