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Background and purpose of a growing number of studies have shown that diabetes and its complications and oxidative stress are strongly linked. Chronic complications of diabetes onset unified theory that mechanisms: activation of the polyol pathway, increased non-enzymatic glycation of protein, protein kinase C activator, hexosamine pathway activation in diabetic complications caused by four common pathway induced by high glucose are essentially superoxide overexpression results. Diabetes is caused by diabetes, liver damage liver function and histological changes of the lesion, are areas of chronic complications of diabetes. Oxidative stress and lipid peroxidation, not only can directly damage the liver cells, inflammatory cells to induce chemotaxis of inflammatory lipid infiltration, also activate liver fat-storing (stellate cells) promote collagen synthesis, resulting in fibers of even cirrhosis. Antioxidants can reduce the production of free radicals or direct quenching free radicals produced by the body, enhance the body's antioxidant capacity. Therefore, the use of antioxidants in order to improve the treatment of diabetes pathophysiology of abnormal body condition, a current treatment of diabetes and its complications is one important way. Variety of antioxidants such as vitamin C, E and α-lipoic acid and other complications of diabetes can have a beneficial impact. Tongluo Recipe (Tongluo Recipe, TLR) is the traditional Chinese medicine from ginseng, leeches, centipede, scorpion, woodlouse and TPG and other components. Modern pharmacological studies have shown that the main active ingredient of ginseng ginsenosides with lowering blood pressure, anti-oxidation, anti-apoptotic effects; leech a diuretic, eliminating the role of protein in the urine, and can improve microcirculation; Eupolyphaga and scorpion anticoagulant, anti- thrombosis; TPG has a reduced concentration of whole blood, inhibit platelet aggregation, reduce platelet moving attached, reducing fibrinogen, and time limit thrombosis effect. Previous studies have shown that this project through TLR through its antioxidant and improve hemodynamics and other effects to reduce diabetic rats concurrent kidney, vascular endothelium, cardiomyocytes, hippocampus and other brain damage. Whether through TLR liver damage diabetes has a therapeutic effect, there is no relevant reports. This experiment used streptozotocin (streptozotocin, STZ) diabetic rat model was prepared to observe its experimental diabetic rats serum liver function, liver fibrosis-related indicators of oxidative stress in liver tissue and liver morphologic changes, aimed explore through TLR efficacy in diabetic liver damage and its possible mechanism. Research Subjects and Methods SD rats for the study, I streptozotocin 60 mg / kg intraperitoneal injection, 1 week after blood glucose ≥ 16.7mol / L for diabetes model was successfully. The diabetic mice were randomly divided into four groups (n = 10): (1) diabetic control group (DM); (2) through TLR small dose group [TL, TLR dose of 0.5g / (kg · d)]; (3) through TLR mid-dose group [TM, TLR dose of 1.0g / (kg · d)]; (4) through TLR high-dose group [TH, TLR dose of 2.0g / (kg · d)]. While taking 10 rats were used as normal control group (CN). Experimental period of 12 weeks. 1 detects the rats body weight, blood glucose, blood lipids [triglyceride (TG), high density lipoprotein (HDL)] 2. Serum liver function [alanine aminotransferase (ALT), aspartate aminotransferase 3 light microscope and transmission electron microscope morphological changes in liver tissue. 4 liver tissue oxidative stress-related indicators [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX) malondialdehyde (MDA)] Result: 1 blood glucose and serum triglycerides were significantly higher (P <0.01), body mass and serum high-density lipoprotein decreased significantly (P <0.01). Meridians prescription treatment after 12 weeks in the high dose group of diabetic rats compared with untreated mice body weight has increased, but not statistically significant; diabetic rats, blood glucose and lipid indicators through TLR did not see significant changes after treatment . 2 diabetic rats complicated by liver damage, liver function indexes ALT, AST and AKP were significantly higher (P lt; 0.01). Meridians prescription treatment after 12 weeks in the high dose group of diabetic rats serum ALT, AST levels were significantly decreased (P lt; 0.05), liver damage a certain degree of improvement. 3 diabetic rat serum liver fibrosis HA and Ⅳ-c levels were significantly higher (P lt; 0.01). Meridians prescription treatment after 12 weeks, the high dose group of diabetic rats HA serum liver fibrosis and Ⅳ-c levels were significantly decreased (P lt; 0.05). 4 light microscopy and transmission electron microscopy showed diabetic rat hepatocytes obvious pathological changes characteristic of diabetes: cytoplasmic glycogen granules increased significantly, increased cytoplasmic lipid droplets, hepatocyte mitochondria matrix becomes fuzzy and dense, coarse endoplasmic reticulum partial degranulation, smooth endoplasmic reticulum expanded. Meanwhile, the diabetic rats with liver fibrosis and pathological changes of microvascular injury: hepatic sinusoid edge periportal hepatic collagen fibers periportal vascular endothelial cell mitochondria cristae missing, some vascular wall thickening, Disse space liver fat storage cell proliferation. Meridians prescription intervention after 12 weeks, diabetic rat hepatocyte cytoplasm decreased glycogen granules, mitochondria and matrix density reduction becomes clear, rough and smooth endoplasmic reticulum morphology close to normal. Diabetic rat liver and liver sinusoids edge periportal collagen fibers reduced periportal vascular endothelial cells and vascular wall thickness near normal, Disse space to reduce proliferation of liver fat-storing cells. Tongluo prescription treatment that significantly improved the pathological. 5 diabetic rat liver tissue antioxidant enzymes SOD, CAT activity were significantly lower (P lt; 0.01); GSH-Px levels also declined, but the difference was not statistically significant; MDA levels were significantly higher (P lt; 0.01) liver tissue is excessive oxidative stress. Meridians prescription treatment after 12 weeks, in the low dose group diabetic rat liver tissue SOD activity was significantly increased (P <0.01); in the high dose group diabetic rat liver tissue MDA levels were significantly decreased (P lt; 0.01), liver tissue oxidative stress levels declined. Conclusions: 1. Diabetic rat serum liver function and liver fibrosis increased, while the characteristic of diabetes liver morphological changes. Tongluo prescription treatment can significantly reduce the blood biochemical abnormalities, significant improvement in liver pathology forms of diabetes liver damage has a therapeutic effect, the therapeutic dose of 2.0g / (kg · d) when a significant effect. 2 diabetic rats increased levels of oxidative stress in liver tissue, meridians recipe can enhance the activity of antioxidant enzymes in liver tissue, removal of lipid peroxides, reduced levels of oxidative stress in liver tissue. 3 clearly lipid disorders in diabetic rats, meridians prescription treatment no significant lowering blood sugar, blood lipids. Therefore, analysis of Tongluo Recipe for the treatment of diabetes, liver damage does not depend on its regulating effect of glucose and lipid levels, and its mechanism and its role of oxidative stress are closely related.
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