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Planktonic virus is free in the water bodies of the various types of virus, and think that is an important ingredient in the microbial community of the body of water, it plays an important role in the microbial loop, biogeochemical cycles, community structure and diversity change. Study of planktonic virus in different waters and different environmental abundance and molecular diversity, you can explore the distribution and ecology of the status of the planktonic virus, to lay the foundation for a better understanding of the biological community structure variation and provide data reference for the environmental assessment and theoretical basis. North Yellow Sea region represents an important type of the the North temperate marine ecological environment, only a few in the area to technology research planktonic virus abundance reported by fluorescence microscopy. Planktonic virus abundance of North Yellow Sea waters fall detection using flow cytometry, and the correlation analysis of of planktonic virus changes in abundance and hydrological parameters. Planktonic virus abundance of various sites in the range of 2.76 × 106 ~ 3.97 × 107 mL-1, with an average of 2.12 × 107 mL-1; horizontal distribution of planktonic virus high value area is mainly distributed in the survey area southeast waters . On the vertical distribution of the water layer planktonic virus abundance was no significant difference. Diurnal variation, the highest value of the abundance of planktonic virus appears in the 30m layer 19:00 time, the lowest values ??appear in the 10m layer 4:00 moment. Pelagic viral abundance in the after 1:00 time peak, a sharp decline, at 4:00 to except the lowest (bottom), there is a peak at 7:00 upward trend from 10:00 to 19:00. Correlation analysis showed that planktonic virus abundance of heterotrophic bacteria, Synechococcus, pico-eukaryotic phytoplankton showed a significant positive correlation, with VBR correlation. Pelagic viral abundance and temperature are correlated significantly negatively correlated with salinity and depth. Algal DNA virus is extremely rich diversity in the ocean, in the country yet to see reports. This is the first use of the DGGE technique survey the northern Yellow Sea, Qingdao offshore algae DNA viruses community diversity. A604 station in the northern Yellow Sea, the B107 the station and C803 station, respectively 10, 8, 8 OTUs (operational taxonomic units), the Qingdao inshore ZQ station 6 OTUs different waters algal DNA virus diversity differences in the northern Yellow Sea is rich, slightly lower in Qingdao inshore diversity. Compared between the three stations in the northern Yellow Sea, The OTUs number and location differences, suggesting that the same sea the different stations algae DNA viral diversity differences. The resulting OTUs the two OTUs there are four stations, which shows there are some common algae DNA virus in the northern Yellow Sea and offshore Qingdao. Based on DGGE patterns intuitively North Yellow Sea, Qingdao offshore algae DNA virus diversity preliminary study finalized virus in one environment composition and taxonomic status needs to combine gene sequencing system tree. Of using PCR-RFLP technique, evolutionary tree of North Yellow Sea algae DNA genetic diversity of the virus family through to build algae DNA virus system. The established North Yellow Sea algae the DNA viral DNA polymerase library, 329 positive clones were randomly picked and RFLP analysis. 18 OTUs belonging to three clusters: Cluster I separate needle Synechocystis virus red tide exclusive Bay the algal virus HaV01 kinship closer, presumably belong to the same virus, needle alga; clusters II and brown algae virus genus FsV closer genetic relationship, presumably brown algae viruses belong to the same genus; clusters Ⅲ as cultured taxa, the taxonomic status needs further study. Not detected in the northern Yellow Sea to the virus is parasitic algae, Chlorella virus genus, a member of the the coccolithophores virus genus and the golden algae virus.
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