Dissertation > Excellent graduate degree dissertation topics show

Isolation, Identification and Detection of Drug Resistance of Duck Escherichia Coli in Zouping Region

Author: LiGang
Tutor: JiangShiJin
School: Shandong Agricultural University
Course: Veterinary
Keywords: duck Escherichia coli serotype drug resistance quinolone gyrA parC
CLC: S852.61
Type: Master's thesis
Year: 2010
Downloads: 133
Quote: 1
Read: Download Dissertation

Abstract


In clinical veterinary, Colibacillosis can cause serverse hazardous, extraordinary avian Colibacillosis. Because this disease has complex clinical symptoms and diversification of pathology characteristic, it is a common disease puzzled by cultivates estate evolutionary. For the past few years, principal disease can basic control by intensive breed, but Colibacillosis can showed the upgrade tendency and it can become one of the chief bacteriosis in cultivate estate.In China, over 50 kinds of serotypes of avian pathogenic Escherichia coli have been reported. In the recent years, duck industry has developed quickly in Zouping region of Shandong province and the serious damage of duck colibacillosis follows naturally. To prevent and cure duck colibacillosis effectively, identification and drug-susceptibility analysis of duck pathogenic Escherichia coli were particularly important. This study includes three parts:Part one: Escherichia coli were isolated from diseased ducks from duck farms in Zouping region of Shandong provinceduring 2009, and 12 Escherichia coli isolates were characterized for O serotypes by serological identification. Four serotypes (O78, O35, O18 and O15) were were identified in the 12 Escherichia coli isolates, and the most common serotypes were O78 and O35 (58.35% and 25% respectively).Part two: Drug susceptibility determinations were used to test the isolated 12 Escherichia coli strains isolated from Zouping region by Kirby-Bauer method recommendation by WHO. The result showed that the Escherichia coli isolates had generally generated resistance to 17 kinds of drugs, highest degree to penicillin, tetracycline and rifampicin (most with inhibitory bacterium loop), higher degree to quinolones, but generally sensitive to amikacin, gentamycin and furadantin. Meanwhile, 83.33% ( 10/12 ) of bacterium had generated resistance to over 9 kinds of drugs.Part three: Owing to the strongest resistance to quinolones, gyrA and parC genes of the 12 Escherichia coli isolates were clond and sequenced. The result showed that mutations of gyrA and parC of most bacterium accord with the results of drug-susceptibility analysis except some ones, which indicated there were more complicated mechanism of resistance to quinolones in duck pathogenic Escherichia coli isolates in Zouping region.

Related Dissertations

  1. Development of Subunit Vaccine of Streptococcus Suis Type 2 and Actinobacillus Pleuropneumoniae,S858.28
  2. 2007-2009 Chongqing Salmonella serotypes prevalent features and gathering food poisoning investigation,R155.3
  3. Studies on the Synthesis of Benzotriazoles and 4-Qquinolones,R914
  4. Study on Types and Active Surveillance of Foodborne Salmonella.spp in Shandong Province,R155.5
  5. Synthesis and Chemical Process of 4-(3-(Tert-Butyldimethylsilyloxy)-1, 1-difluoropropan-2-yl) Morpholine,TQ463
  6. Pathogenic Vibrio parahaemolyticus analysis and internal standard PCR assay evaluation,TS254.7
  7. Studies on Etiological Characteristics of Haemophilus Parasuis Isolated from Jiangxi Province,S852.61
  8. Study on the Pathogenicity of Ureaplasma Urealyticu Serotype 3 and 8 in Female Rabbits’s Reproductive Tract,R711.3
  9. Isolation and Resistance Analysis of Avian Pathogenic E.coli in Jining Town,S852.61
  10. Study on Quinolone Resistance Gene of Mycobacterium Tuberculosis,R378
  11. Application of Flow-injection Chemiluminescence in Analysis of the Quinolone Drugs,R914
  12. Biological Function of Pili-like Protein SSU2101 in Streptococcus Suis Serotype 2,S852.611
  13. Biological and Genetic Characterization of Streptococcus Suis Type 2 Isolates from Slaughter Pigs,S852.61
  14. Development of a Serotype Specific PCR Assay for Shigella Flexneri and Analysis of a Serotype-converting Bacteriophage SfX,R440
  15. Isolation and Identification of Goose Escherichia Coli in Some Areas of Heilongjiang Province,S852.61
  16. The Studies on the Mechanism of Plasmid-mediated Quinolone Resistance of Clinical Isolates in Escherichia Coli,R378.21
  17. Anti-tumor Effects of Alkaloids from TCM Sophora Plants,R285
  18. Research on Design of the MU-MIMO Adaptive Algorithm in TD-HSPA+ System,TN929.5
  19. Synthesis and Activities of the 7-azepanylqunolones,R914
  20. Study on Quinolones-resistant Mechanism of ESBLs Producing and Non-esbls Producing Klebsiella Pneumoniae,R446.5
  21. Direct Oxidative Coupling of N-actylindoles with Anisoles Using Hypervalent Iodine Reagents and Palladium-catalyzed Sequential Cyclization/coupling Reaction,O643.32

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Animal Microbiology ( Veterinary Microbiology, ) > Pathogenic bacteria
© 2012 www.DissertationTopic.Net  Mobile