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Study on Separation and Purification Method of High-Purity Fructooligosaccharide
Author: WuJinLiang
Tutor: LuJiaJiong
School: Guangxi University
Course: Sugar works
Keywords: Fructooligosaccharides Activated Carbon Nanofiltration Separation and purification Gel chromatography
CLC: TS245
Type: Master's thesis
Year: 2008
Downloads: 459
Quote: 4
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Abstract
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Oligofructose has a significant role in promoting the growth of bifidobacteria in the human gut , and protect the liver , improve immunity and other physiological functions , widely used in food and health products industry . However, large - scale production of fructooligosaccharides product is a mixture of various components , purity is not high to limit the public 's sense of identity of its physiological functions . Due to technical constraints, the oligosaccharide products for domestic market shortage of high - purity , which makes purification of fructooligosaccharides has great realistic significance . This topic is preliminary research decolorization effect of oligofructose syrup . The results showed that 70 ℃, pH = 3.8, 1.4% carbon dosage fructooligosaccharides decolorization activated carbon decolorization 50min . The new ecological calcium carbonate Law for fructooligosaccharides decolorization effect is not ideal . Also studied the use of membrane separation method after the decolorization oligofructose two nanofiltration and one reverse osmosis , and the separation of a purity of more than 90% and a purity of more than 50% of the the oligofructose product I and product II , as well as glucose and sucrose mixed product III . Product Ⅰ Although the yield is not high , but for subsequent gel chromatography the fructooligosaccharides single component provides a source of raw materials , but also for the Bio-gel P-2 gel chromatography separation has laid a good foundation . Polyacrylamide gel P-2, when the flow rate of 0.12mL · min -1 sup>, 120 × 1cm ID, the injection volume of 0.5mL , high purity oligofructose as raw material initially been kestose , the cane fruit four sugar and cane fruit pentasaccharide single component , the higher the purity of raw materials , the higher the purity of the single components . Dextran LH-20 in pure water as the mobile phase separation of the single components of the fructooligosaccharides undesirable . Use of KS and CS series gel sugar column , using water as the mobile phase , three - column series method can effectively separate fructooligosaccharides , the research oligosaccharides substance provides a new, environmentally friendly energy separation method .
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CLC: > Industrial Technology > Light industry,handicrafts > Food Industry > Sugar industry > A variety of sugar production
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