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Luteolin (luteolin,) is a flavonoid compound, has a variety of biological activity, have a higher content in peanut shells. Using ethanol as extraction solvent extracted from peanut shells luteolin optimize the extraction process, using a variety of methods to separate and purify, and studied the the luteolin initial EP-1 and the purified product of EP-2 antibacterial activity of purified material. The main findings are as follows: 1 under the same conditions three extraction methods luteolin yield: water extraction> Microwave Extraction> ultrasonic extraction, that is, 0.876%, 0.801%, 0.430%; luteolin water extraction resulting dry matter hormone content of 9.85%, the microwave extraction dry matter content of 15.18%; determine the optimal extraction process as follows: microwave power 900W, 80% ethanol, extraction time 70s, 1:20, the extraction rate is 82.66%. High performance liquid chromatography peak area ratio method to analyze the purity of the purified and possible impurities, ethyl acetate extraction can be detected in the water-soluble ingredients were enriched water precipitation method can significantly reduce the water-soluble ethanol solution sexual ingredient, anhydrous ethanol dissolved dry matter luteolin and a fat-soluble components were enriched thin layer to expand the system can achieve a high degree of purification of the sample. Macroporous resin purification method using sample -30% ethanol and 70% ethanol 70% ethanol sample higher purity of the product obtained in 30% ethanol; anhydrous ethanol-like method, the amount of sample handling, the target mainly concentrated in 70% ethanol fractions; purification process to determine the kind of anhydrous ethanol, ethanol gradient elution of 70% Break collected fractions. 4 of the initial purified (EP-1) on the test bacteria have a certain inhibit or kill, has a broad-spectrum antibacterial properties, especially effective in killing bacteria, the role of fungi poor. Antibacterial activity within the test range of EP-1 with the concentration and time into a positive correlation at the same time, the higher the concentration of the extraction liquid, the higher the inhibitory rate; extract at the same concentration, the longer duration of action, suppressing the higher rate of bacteria. 5.EP-2 antibacterial activity was significantly higher than the EP-1, and highlights the antibacterial characteristics of phenolic compounds. Its antibacterial activity at the same concentration of sorbic acid and sodium nitrite, Bacillus subtilis and Staphylococcus aureus antibacterial activity of sorbic acid and sodium nitrite had no synergistic or antagonistic; inhibition of brewer's yeast and Asian sodium nitrate synergistic effect; incubation temperature, pH inhibitory effect of the EP-2's have a greater impact. Biological microscope bacterial form in the liquid after characterization, the results show: the structure and function of bacterial outer membrane and cell wall is damaged, a large number of bacterial debris blocking nutrients transport within the cell cytoplasm wall separation , resulting in the leakage of their contents and play an inhibitory effect.
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