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Characterization of Fusarium sp. from Electrolytic Manganese Residue and the Study on Its Leaching Ability of Manganese

Author: LiHuanLi
Tutor: LiXiaoMing
School: Hunan University
Course: Environmental Engineering
Keywords: Fusarium sp. Bioleaching Manganese waste Mechanism
CLC: X172
Type: Master's thesis
Year: 2009
Downloads: 55
Quote: 0
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Abstract


Bioleaching is a use of microorganisms to extract minerals from valuable metals economic approach, especially for dealing with lean ore, ore and waste rock sheet and low cost, small investment, low energy consumption, little environmental pollution and other advantages . In biological metallurgy, for efficient leaching Strains and mechanism of great significance. Peng Xiaowei and other waste from electrolytic manganese manganese screened a strong resistance to fungal A5, and its preliminary identification. In this study, by amplifying 26S rDNA D1/D2 region sequences, A5 further identified as Fusarium fungus genus one kind. In order to study the role of manganese in the leaching residue, from manganese slag and Fusarium sp. Starting characteristics, through its leaching manganese flask experiment conditions were optimized, also investigated the Fusarium sp. Manganese slag after leaching manganese morphological changes, and its ability to dip manganese were studied preliminarily discussed Fusarium sp. dip manganese mechanism. Screened soil from manganese slag separation of manganese strong resistance to a fungal A5, has its morphological characteristics were studied. Its hyphae elongated, loose colonies, colony surface with green, blue, black, orange and different colors, the medium color on both sides there are some differences. Based on 26S rDNA, sequencing results showed that the bacteria belonging to the genus Fusarium is one kind. The use of Fusarium sp. Carry manganese slag shake flask leaching tests, the test found that the pulp density of 10%, the highest rate of leaching of manganese, when the slurry concentration is greater than 10%, manganese leaching rate declined; manganese leaching rate increased with the increase over time , 30h reaches a maximum; increase inoculum size, has little effect on the leaching rate of manganese. Fe2 and Fe3 adding manganese leaching rate can be improved. Using the optimized BCR (European Community Bureau of Reference) sequential extraction scheme for Fusarium sp. Before and after leaching manganese morphological analysis carried out to study the leaching rate of its manganese and manganese before and after leaching characteristics of the morphological changes. Also investigated three kinds of extraction agent EDTA, HNO 3 and CaCl 2 on the extraction efficiency of manganese and manganese extracted morphological changes found Fusarium sp. Dip for manganese the ability to take more significant, three days after the manganese leaching rate of 56.5%, is conducive to the recovery of manganese from manganese slag. Three kinds of extraction agent for manganese leaching effect of EDTA gt; HNO 3 gt; CaCl 2 , the average extraction efficiencies were 50.0%, 28.8% and 21.2%. Before and after leaching, acid dissolved manganese more significant changes in the proportion of the acid dissolved manganese is relatively easy to leach forms. Fungi leaching process the biological acid leaching is the most important agent. Fungal bioleaching process, not just its biological acid involved in the metabolism of chemical reactions, the microorganism itself also played an important role. By examining the different concentrations of nitric acid, citric acid and oxalic acid (0.1M and 0.5M) dip manganese capabilities and Fusarium sp. Manganese leaching process medium pH changes, the Fusarium sp. Dip manganese mechanism were studied. At the same time before and after leaching Fusarium sp. Were scanning electron microscopy showed that, Fusarium sp. Produce biological acid metabolic process and participated in the reaction, Fusarium sp. Manganese leaching process itself also played a role in the adsorption.

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