|
Carried out cinnamaldehyde and cinnamic acid biodegradation and its transformation products to track and analyze on the basis of the established method of qualitative and quantitative analysis of complex biological reaction system, the cinnamic aldehyde, cinnamic acid as substrate strain selection and transformation other aspects of the research, the research results are as follows: (1) the use of RP-HPLC simultaneous analysis of acetophenone and cinnamic acid analysis method to determine the chromatographic conditions: mobile phase of methanol: water: ice acetate = 55:45:0.05; detection wavelength was 246nm, and a flow rate of 1.0 mL · min -1 . In optimum conditions, acetophenone, cinnamic acid linear equations were: Y = 32017X-6298.6; Y = 11286X-4508.7 and precision experiments and recovery experiments. (2) to study the cinnamic aldehyde, benzaldehyde and benzoic acid UV absorption spectral characteristics, the establishment of a three-component UV spectrophotometry content, results show that cinnamic aldehyde and benzaldehyde at 228nm and 256nm wavelength build a simple and rapid quantitative analysis of the mixture such as absorbency, the use of absorbent points, given the relationship between the concentration and the absorbance of each component, respectively-C cinnamic aldehyde = (A 311 -0.0084) / 0.0424; C the benzene, formaldehyde = (A 256 -0.0005) / 0.0622-C cinnamic aldehyde; the C benzoic acid = (A of 228 0.0057) / 0.0611-C the cinnamic aldehyde -C benzaldehyde . Cinnamic aldehyde linear range :0-1 8.03μg · mL -1 , benzaldehyde linear range :0-13 .08 g mL -1 , benzoic acid linear range :0-9 .233 μg · mL -1 . The method precision (RSD) were lt; 1% RSD of stability are lt; 1%. Recovery tests using the standard addition method, determination of sample recoveries were between 99% -101%. (3) experiment with some of the soil samples in the peak forest, peak oil plant in Guangxi, Yu Lin Pingnan collection, a total of 122 soil samples, the strain of separation and transformation of cinnamic aldehyde benzaldehyde experiments, screened 186 single bacteria, including the ability to transform 6, from which to select a relatively high ability to transform a bacteria (bacteria # ), the conversion conditions optimization experiments. The ability to transform higher 5 of # bacteria Optimization of carbon sources, nitrogen sources, temperature, fermentation medium initial pH, amount of liquid. The results show that, with glucose as the carbon source, beef extract as nitrogen source, the temperature maintained in the 30 ° C, initial pH of 5.0, the liquid volume of 100mL best conversion conditions, benzaldehyde content of up to 5.24%. (4) Filter to a plant height of the ability to transform strain, it will be efficient, selective degradation of cinnamic acid acetophenone. The Burkholderia genus identified bacteria Vietnam cepacia (Burkholderia vietnamiensis). (5) Vietnam cepacia conversion of cinnamic acid to generate acetophenone, optimized translational research, optimal conditions: sucrose as a carbon source, concentration of 2g · L . -1 peptone as nitrogen source concentration of 2.5g · L -1 30 ° C, fermentation 2.5d, transforming 2d, pH 9.0 in the transformation process in the best conversion conditions, conversion of 0.5 g of cinnamic acid After the substrate, the content of the acetophenone of up to 3.24g · L -1 , cinnamic acid, the conversion rate was 97.2%.
|