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A Preliminary Investigation on Regulation of Mitochondrial Motility by Hydrogen Peroxide in Myoblast

Author: DiKeMin
Tutor: ZhangYong
School: Tianjin Institute of Physical Education
Course: Human Movement Science
Keywords: Myoblast Mitochondrial motility Hydrogen peroxide Energy metabolism [Ca2+]i
CLC: Q244
Type: Master's thesis
Year: 2010
Downloads: 20
Quote: 0
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Abstract


The mitochondria is organelles of movement and highly dynamic structure, reactive oxygen species is a regulating center of variety important cellular signal transduction pathways, the purpose of this research was to investigate changes of mitochondrial movement in myoblast under model of mild oxidative stress, and initially explore the possible relationship between the exogenous H2O2, mitochondrial movement and energy metabolism. It is observed that mitochondrial movement of the myoblasts under the model of mild oxidative stress by laser scanning confocal microscope. The our results showed that showed two kinds of mitochondrial movement mode– motility and local swing in primary myoblasts and C2C12 cells; [Ca2+]i declined to regulate transient increase of mitochondrial movement in myoblasts; the exogenous H2O2 of appropriate concentration may affect mitochondria motility in myoblast by regulation [Ca2+] i ; the miro gene expression was increased significantly in myoblast cells by 10 and 100μM H2O2 treatment of myoblasts at different times （p<0.05, p<0.01）; miro protein content was also increased significantly （p<0.05, p<0.01）; the myoblast cells showed oxygen uptake rate are a transient decline on H2O2 of exogenous high concentrations, they are gradually increase with the increment H2O2 concentration of exogenous treatment that the amplitude of step down and persistence time of the oxygen consumption rate of cell; and found that H2O2 of 100μM, 1 mM and 10 mM can induce significantly decreased ATP generation of myoblast cells by 2 hours processed, decreased by 17%, 38% and 50 % （p <0.01）. This indicates that the H2O2 of appropriate physiological concentrations or low concentrations can be used as cell signaling molecules, on the one hand, which regulate gene transcription on the one hand; on the other hand, H2O2 may firstly regulate the activity of calcium channels, triggering skeletal myoblasts SR and mitochondria on Ca2+ uptake, resulting in [Ca2+]i temporary, slight decline, and then appeared to increase mitochondrial movement, regulation of mitochondrial redistribution.

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CLC: > Biological Sciences > Cell Biology > Cell morphology > Intracellular membrane system
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