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Droplet microfluidic chip is emerging in recent years, biochemical analysis platform . Because of its high efficiency, high -throughput , low consumption advantages , droplet microfluidic chip is used in many scientific areas, such as enzyme kinetics , cell analysis , enzyme immunoassays , DNA analysis. In this paper, we designed a PDMS-based materials Focused fluid droplet micromixer chip , the use of liquid droplets in the winding channel convection caused by motion , carried out research activity and single cell experiments wrapped inside the droplet . We optimized the first chip structure, it has a higher mixing efficiency . Optimize the use of the chip , we explored the droplet micromixer mixing manifold , and use blue ink mixing experiments with pure water and the qualitative and quantitative chemiluminescence method confirmed the structure of the chip , compared with the previous design on the mixing efficiency. After determining the droplet microfluidic chip performance as a mixer in the mixing area , we use horseradish peroxidase (HRP) / luminol chemiluminescence system demonstrates the droplet micromixer for enzyme activity assay in the application prospects. Through line dilution method to study the same concentration of anti-IgG HRP and HRP enzyme activity differences. The results showed that when HRP linked with anti-IgG , its activity than HRP reduced by 4 times. In addition , the use of the chip size of the droplets formed controllable characteristics, we apply it in terms of the cell wrap , wrap them in a cell for the current problems , such as cell injection method , cell concentration , we did some exploring . We explored the different concentrations , droplets cell parcel situation, choose the most suitable package concentration for a later analysis of single cell within the droplets and lay a solid foundation.
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