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Study of Population Genetic Structure of House Mouse MUS MUSCULUS CASTANEUS in Shanghai

Author: ZhuWangSheng
Tutor: XiaoJunHua
School: Donghua University
Course: Applied Chemistry
Keywords: Mus musculus Population genetic structure Genetic diversity Mitochondrial single nucleotide polymorphisms Short tandem repeat
CLC: Q953
Type: Master's thesis
Year: 2008
Downloads: 15
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Abstract

In this thesis, the genetic polymorphism molecular markers to study the Shanghai region Mus musculus population genetic structure and its relationship with the geographical distribution of molecular markers and the theoretical basis for Mus musculus resource utilization. Autosomal short tandem repeat the (autosomal short tandem the repeat autosomalSTR) and mitochondrial genome coding region single nucleotide polymorphism molecular markers (mitochondrialsingle nucleotide polymorphisms, mtSNPs) is used for the Shanghai five different counties: Chongming (CM), the Qingpu (QP), Jinshan (JS), Jiading (JD) and Songjiang (SJ), Mus musculus population genetic polymorphism distribution and genetic structure of the investigation. The research topics include: (1) by fluorescence - conformation sensitive gel electrophoresis (fluorescence-based conformation sensitive gelelectrophoresis, F-CSGE) and direct DNA sequencing technology, the initial screening Shanghai Mus musculus mitochondrial coding region single nucleotide state the molecular markers (mtSNPs); (2) application of LDR-sequencer typing techniques from the Shanghai five different counties 112 small norvegicus mtSNPs of typing; (3) by fluorescent PCR technology from Shanghai five different counties 112 Mus musculus 5 the nuclear genome STR typing. The main results are as follows: the the F-CSGE in 20 samples (kb length of the coding region of each of the four regions 5 Mus musculus) mitochondrial genome mtSNPs23, an average of about 0.2 kb exist a mtSNPs sites, including newly discovered mtSNPs15. Select 7 tag SNPs (tag SNPs), of the seven mtSNPs sites in the the Shanghai area 112 overall sample distribution rule as follows: L350 (T: 82%, C: 18%), L366 (C: 88%, T : 12%), L543 (A: 92%, G: 8%), L572 (T: 79%, C: 21%), L716 (T: 77%, A: 23%), L953 (T: 89% , C: 11%), L964 (G: 78%, A: 22%). Among them, the the L572 C GT three mtSNPs sites; T, L716 A GT; T and l964 A GT; distribution law of G there are regional differences. Network software, Mus musculus 7 label mtSNPs of in the Shanghai area groups to build nine haplotypes, H5 single haplotype (TCATTTG) Shanghai ancestors haplotype (ancestor Haplotype). H5 single-of haplotypes (TCATTTG) and Mus musculus subspecies Musmusculus molossinus in Japan (TCATTTG), and existing laboratory inbred Mus musculus (TTATWTG (W: A / T)) and the European Mus musculus subspecies different Mus musculusdomesticus (TCATATG). Added new evidence for the of existing Mus musculus worldwide migration routes hypothesis. The autosomal STR mtSNPs show that the larger Shanghai Mus musculus population genetic differentiation. Groups based on STR data to build the tree indicates that Songjiang small norvegicus groups as early as in other regions differentiation independent one; mtSNPs data indicates that the the Chongming Island Mus musculus groups and the mainland groups exist different maternal ancestor, showing the isolated groups features. The island of Chongming County a short time, the relative isolation of the relevant location. System based on two types of genetic markers constructed Shanghai Mus musculus groups phylogenetic tree there are some differences, genetic information related to differences with the autosomal STR mtSNPs genetic stability of difference and two types of genetic markers included. As autosomal STR the stability below mtSNPs and contains two sets of paternal and maternal genetic system information, mtSNPs contains only maternal inheritance system information. In short, Shanghai Mus musculus groups rich in genetic diversity, genetic distance and physical distance between different groups is positively correlated, Chongming Island group is a relatively isolated groups. Mus musculus resource rich genetic diversity for the use of complex disease-related genes high-resolution positioning research offers the possibility to make up for the experimental strains of mice lack genetic polymorphism. Mus musculus resources for further use, this study provides a theoretical evidence and practical basis.

Study of Population Genetic Structure of House Mouse MUS MUSCULUS CASTANEUS in Shanghai

Abstract

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