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Hemoglobin Immobilization and peroxidase activity

Author: WangLiXing
Tutor: MaoLuYuan
School: Zhengzhou University
Course: Materials Science
Keywords: Immobilized hemoglobin Nanomaterials Enzymatic polymerization Peroxidase activity O-phenylenediamine
CLC: Q814
Type: Master's thesis
Year: 2009
Downloads: 85
Quote: 0
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Abstract


The enzyme-catalyzed reaction and mimetic enzymes supramolecular chemistry , the \Hemoglobin (Hb) and horseradish peroxidase ( HRP ) have the same active center , we study the peroxidase activity . In order to improve the utilization of hemoglobin , we studied the hemoglobin immobilized . Prepared by glutaraldehyde crosslinked hemoglobin molar ratio of the cross - linked hemoglobin samples , with the atomic force microscope of the crosslinked hemoglobin after changes in the size , with the increase in the molar ratio of glutaraldehyde and hemoglobin participation deposit linking of Hb molecules increases, the molecular size increases ; Trinder reaction to detect the peroxidase activity, and found that the cross -linked hemoglobin peroxidase activity has decreased to some extent than the uncrosslinked hemoglobin . Polyvinyl alcohol prepared radiation SYSTEM - acrylic hydrogel embedding hemoglobin, the degree of polymerization of polyvinyl alcohol , acrylic acid content , the irradiation dose on the swelling properties of the hydrogel . The design and synthesis of nano-silver - containing polyvinyl alcohol - acrylic hydrogel and study the conversion rate of irradiation during the restore process silver and hydrogel sustained release of silver in the water speed . Embedding hemoglobin peroxidase activity . Hemoglobin and hemoglobin immobilized in the aqueous phase micellar solution selective catalytic polymerization of o-phenylenediamine , IR, NMR , fluorescence , cyclic voltammetry , GPC and X-ray diffraction method on the structure and properties of the polymer were characterized . The thermal analysis results show that the micellar solution with hemoglobin and synthetic polymers cross - linked hemoglobin catalytic good heat resistance at 350 ℃ , the weight remains more than 85% . Poly - o-phenylenediamine morphology with an atomic force microscope observation on the mica substrate , the polymer particles are uniformly dispersed in a smaller range ( 625nm x 625nm ) observation found that the polymer particle diameter of less than 100nm , which we use matches the particle size of the X -ray diffraction method .

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