Dissertation > Excellent graduate degree dissertation topics show

Functional Analysis of MdSPDS1 and Primary Study on Transgenic Populus Tomentosa Carr.

Author: LiuZuoZuo
Tutor: ZhangZhiYi；PangXiaoMing
School: Beijing Forestry University
Course: Tree Genetics and Breeding
Keywords: MdSPDS1 polymines Populus tomentosa Carr. salt tolerance transformation
CLC: S792.11
Type: Master's thesis
Year: 2008
Downloads: 101
Quote: 2
Read: Download Dissertation

Abstract


The problem of the salty soil has represented some of the most significant constraints to agricultural and forestry productivity. It has become an important topic and hotspot on the researching of the future development in agriculture and forestry that how to make use of the large quantity of salty soil and salty water resources. Polyamines play an important role in plants to enhance environmental stress tolerance. So, functional analysis of MdSPDS1 gene which is encoding Polyamines biosynthesis has been carrying out. And transgenic Populus tomentosa Carr. with MdSPDS1 was abtained. The major results and conclusions are described as follows.(1) MdSPDS1 was transferred to Nicotiana tabacum by Agrobacterium–mediated, and the expression of MdSPDS1 gene was analyzed by Real time Quantitative PCR. The contents of three main polyamines -- Put、Spd、Spm were detected by HPLC. The result showed that the Spd levels in transgenic plants were 2-3 fold when compared to untransgenic plants.(2) It was found that transgenic plant D14 which has high expression of MdSPDS1 could live under 400mM NaCl stress. Under this stress, contents of polyamines were detected to learn the changes of three main polyamines. The results illustrated that the increase of Spd content and (Spd+Spm)/Put played an important role in enhancing tolerance to salt stress. On the other hand, it was found that the ability of resistance to TMV in D14 was higher than WT, and PAs levels altered by TMV infected. Put levels in WT were enhanced more than 5-folds when compared to healthy control; while Spd levels in D14 were enhanced 4-folds. The result showed that increase of Spd was one of the reseaons of enhancing plant resistance to TMV.(3) The primary transformation system was established in Populus tomentosa. The integration of the MdSPDS1 gene into plant genomes of transgenic Populus tomentosa was confirmed by PCR and Southern blot analysis. The expressions of MdSPDS1 gene and polyamines levels were also detected. The result showed that the Spd levels in transgenic plants were 1.65-folds when compared to untransgenic plants. It was also found that transgenic Populus tomentosa could live under 200mM NaCl stress.

Related Dissertations

  1. Rebellion of an and Shi and Poetic Style Evolution from Kaiyuan to Tianbao,I207.22
  2. Research of Wavefront Transformation Based on Liquid Crystal Spatial Light Modulator,TN761
  3. Service Model Driven Architecture (SMDA) Oriented Modeling Techniques and Tool Implemention,TP311.52
  4. Chinese television media discourse paradigm of the evolution of the multi-angle analysis,G220
  5. Rural Energy Transformation and the Benefits of Ecological Environmental,S210.3
  6. Improvement of the Resistance of Nicotiana Tabaccum to Virus by RNAi and Initial Establishment of Genetic Transformation System of L.regale,S435.72
  7. Xu Peng age of academic thought and clinical experience medication regularity of the treatment of chronic gastritis,R249.2
  8. Based on statistics of the lognormal distribution heteroscedasticity model inferred,O212.1
  9. Transformation of RdreBlBI Gene Drived by Rd29A in ’Benihope’ Strawberry,S668.4
  10. Functional Analysis of an Sclerotinia Disease Resistance Gene,S435.654
  11. The Effects of Overexpression of OsSsr1 on Stress Tolerance in Tobacco,S572
  12. Biological Effect of Rare Earth Element and Genetic Transformation on Arnebia Euchroma(Royle) Johnst.Cell,S567.239
  13. Study on Structral Analysis & Control Method of Generalized Ensemble,N945.1
  14. Improvement in Electroporation Transformation Efficiency of Gene for Bacillus Subtilis with Trehalose,Q78
  15. Cloning 1Dx5 Gene from the Xinjiang Wheat Variety and Constructing Its Expression Vector,S512.1
  16. Agrobacterium-mediated Transformation of GmWRKY21 into Soybean,S565.1
  17. The Genetic Transformation and Functional Analysis of GmFtsH,S565.1
  18. Mechanisms of Alleviating Effect of Seed Soaking with Soybean Isoflavones on Glycine Max, Salt-born Glycine Soja and Their Hybrid Seedlings under Salt Stress,S565.1
  19. Cloning of Proteinase Inhibitor Gene CYS and API from Wild Potatoes and Preliminary Study of Potato and Tobacco Transformation with Spcys,S532
  20. Construction of Expression Vector and Genetic Transformation in Phalaenopsis of Phalaenopsis Floral Meristem Identity Gene LFY,S682.31
  21. Study on Transformation of Cut Chrysanthmemum with DdICE1 Gene,S682.11

CLC: > Agricultural Sciences > Forestry > Forest tree species > Broad-leaved trees > Young
© 2012 www.DissertationTopic.Net  Mobile