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Tissue Culture of Talinurn Paniculaturn

Author: DengXiaoXiang
Tutor: ZhangHuiYing
School: Guangxi University
Course: Crop Genetics and Breeding
Keywords: Talinum Rapid Propagation Callus Morphological abnormalities plants
CLC: S567.51
Type: Master's thesis
Year: 2008
Downloads: 162
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Abstract


Test to of Talinum seed and axillary or terminal bud, adventitious buds induced adventitious buds, callus induction, callus, plantlets strong sprout and take root;, and the morphological changes of the plantlets The investigation and analysis, the initial set up the Talinum rapid propagation system, test results showed that: 1. taken Daejeon robust growth Talinum stem with axillary bud routine sterilization, sterile plantlets sterile plantlets different hormones different carbon sources proliferation the trials and differentiation resulting regenerated plantlets the increased reproduction subculture, determine proliferation rate highest proliferation of plantlets formulations are the the MS NAA 0.5mg / L 6-BA 3.0mg / L consumption the sugar 3% , 256.7% and 310%. 2. Sterilized the Talinum seed collected in the field to obtain a test-tube seedlings in different parts of seedlings as explants, callus induction test to determine the best explants induced callus leaves, callus organization's induction rate is relatively high, while a small amount of bud differentiation, to 43.3%. When adding different concentrations of silver nitrate, the differentiation rate increase when silver nitrate concentration of 5.5mg / L, the differentiation rate increased to 76.7%, 33.4% higher than the recipe without adding the induction formula MS 2,4 -D 1.0mg / L NAA 0.5mg / L AgNO 35.5mg / L . Conducting trials of different explants callus, the choice of the callus induced by the blade subculture to the 3rd generation differentiation test bud differentiation rate is the most appropriate formula MS 6-BA 3.5mg / L , the differentiation rate of up to 90.0%. The tube seedlings seedlings, rooting test. The results show that: the formula MS BA 2.5mg / L the MET 1.0mg / L suitable for in vitro sprout Miller, culture plantlets thick, dark green leaves, the robust test-tube seedlings into 1/2MS NAA 1.5 mg / L rooting medium rooting rate of 100%, the number of the highest average per plant rooting 10.5 / plant. 4. Of tube seedling morphology survey results showed that: a higher rate of morphological abnormalities in differentiation medium and strong seedling medium for 20.9% and 20.6%, respectively, the tube seedlings after following the generation and rooting culture, morphological abnormalities were 16.3% and 12.9%. Rooting culture can make the plant morphology abnormal rate of decline. Plantlets were subcultured using leaves as the explants regenerated plants average morphological abnormalities lowest rate of 26.8% and 31.3% of the terminal bud to stem segments regenerated plants average rate of morphological abnormalities. To sum up: by Talinum tissue culture, has been established Talinum rapid propagation system. Talinum explants rapid propagation of plantlets were the basis for the realization of the theory of the plantlets large-scale industrial production or tissue culture methods in the future to provide reliable chemical mutation breeding.

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