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Approach on the Function of Nanometer TiO2-coated PMMA Intraocular Lenses

Author: CaiLiMei
Tutor: WengJingNing
School: Fujian Medical
Course: Ophthalmology
Keywords: Lens epithelial cells Blood platelet Bacterial IOL Nano - titanium dioxide Surface modification Surface properties
CLC: R779.66
Type: Master's thesis
Year: 2008
Downloads: 48
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Abstract


Objective: A Comparative Study of nano-titanium dioxide (Titanium Dioxide, TiO 2 ) photocatalyst film-modified poly (methyl methacrylate) (polymethyl methacrylate, PMMA) intraocular lens (intraocular lens, IOL) with unmodified TiO 2 film PMMA IOL surface properties for future experimental and clinical applications provide a theoretical basis. Methods: In the main wavelength of 365nm, a wave UV (ultraviolet a, UVA) as the excitation light source (the average light intensity 382μW/cm2). The experiment is divided into two parts, the first part: (1) to the contact angle of the sample TiO 2 / PMMA IOL characterization; ② cell adhesion experiment: two sets of crystal pre UVA irradiation at different times (0, 20, 40min), respectively, is fixed to the bore of a 24-well culture plate, and then inoculated the 20μL concentration of 2 to 3 × 104 cells / mL cell suspension culture 3H succeeding HE staining to observe its morphology, and were counted under microscope the the IOL surface of lens epithelial amount of cell adhesion; ③ The cytotoxicity experiments: cells ibid culture the 5h, TUNEL staining morphology and image analysis using Image-Pro □ Plus software evaluation the IOL surface of cells apoptosis rate; ④ calculate and analyze compare cells in two sets of crystal surface the adhesion of the free energy. Second part: (1) platelet adhesion test: using the the conventional centrifugal prepared platelet rich plasma (platelet-rich plasma, PRP), then the two groups of crystals after different time of pre-irradiation of UVA (0,10,30 min), fixed on 24 well culture plate hole, each costing inoculated with 20μL PRP, culture 2h remove Reiter staining observed morphology, to use the software evaluation IOL surface platelet adhesion rate. ②) kill bacteria experiment: two groups of crystals pre UVA irradiation at different times (0,10,30 min), were placed in the wells, each well was inoculated with 2 mL concentration of 1 × 105cfu/mL Staphylococcus aureus and colon bacilli culture the 2h out after Gram staining observed under an ordinary optical microscope to evaluate the survival rate of the the IOL surface of S. aureus software; (3) analysis and comparison of bacteria in the two sets of crystal surface adhesion freedom. Results: The first part: ① With UVA pre-irradiation time extend, TiO , 2 / PMMA IOL surface contact angle decreases gradually hydrophilicity statistically significant difference (P lt; 0.05). ② cell adhesion assay, UVA pre-irradiated with different time periods, the lens epithelial cells gradually reduced TiO 2 / PMMA IOL surface adhesion compared to PMMA IOL difference was statistically significant (P lt ; 0.05). The ③ cytotoxicity experiments, UVA 20 minutes after the pre-illuminated TiO 2 / PMMA IOL surface you can see more cells were apoptotic morphology, and with pre-according to the extension of time, the rate of apoptosis increased with PMMA IOL There was significant difference (P LT; 0.05). The ④ cells in the TiO 2 / PMMA IOL surface adhesion free energy △ Fadh> 0, PMMA IOL surface △ Fadh lt; 0, thermodynamics the known TiO 2 / PMMA IOL surface more conducive to cell adhesion. Second part: UVA pre-irradiated with different time periods, platelets and bacteria gradually reduced TiO 2 / PMMA IOL surface adhesion volume compared with the PMMA IOL difference was statistically significance (P lt; 0.05). UVA pre-irradiated 30min, TiO lower 2 / PMMA IOL surface of platelet adhesion rate and bacterial survival; Wright's and Gram staining was observed in platelets and bacteria in the TiO 2 < / sub> / PMMA IOL surface are was single scattered in PMMA IOL surface, but the majority of clusters gathered. Similarly, the bacteria in the TiO 2 / PMMA IOL surface adhesion free energy △ Fadh> 0 PMMA IOL surface △ Fadh lt;, seen from the viewpoint of thermodynamics the TiO 2 / PMMA IOL surface is not conducive to bacterial adhesion. Conclusions: (1) experiments with TiO 2 / PMMA IOL after the TiO 2 film modified surface water contact angle smaller hydrophilic enhanced; and with UVA excitation time prolonged of TiO 2 / PMMA the IOL surface water contact angle decreases, hydrophilicity gradually increased. (2) TiO 2 / PMMA IOL after UVA excited, the human eye lens epithelial cells, platelets, and Staphylococcus aureus and Escherichia coli in surface adhesion and growth has been inhibited. (3) surface adhesion free energy calculation results show that the human eye lens epithelial cells, Staphylococcus aureus and Escherichia coli in TiO 2 / PMMA IOL surface adhesion free energy significantly improve, which is not conducive to adhesion.

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CLC: > Medicine, health > Ophthalmology > Eye surgery and surgery > Cataract surgery
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