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Experimental Study on the Inhibition of Corneal Neovascularization with Recombinant Human Endostatin

Author: LiWeiYi
Tutor: GaoXiaoWei
School: Shihezi University
Course: Ophthalmology
Keywords: Recombinant human endostatin Corneal neovascularization
CLC: R779.65
Type: Master's thesis
Year: 2008
Downloads: 48
Quote: 0
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Abstract


Objective: To study of recombinant human endostatin (recombinant human endostatin, rHES) inhibition of rat corneal neovascularization induced by alkali burn . Methods: 48 Wistar rats were randomly divided into four groups : group A ( 12 eyes) in the control group not treated ; B group ( 12 eyes) : double corneal alkali burn after bulbar conjunctiva injected with saline 0.05ml every other day ; C , D group ( 12 eyes ) : the application after double corneal alkali burn 100mg/ml recombinant human endostatin eye , 4 times / day and subconjunctival injection of 0.05ml every other day treatment. Observed at each time point in each group cornea and corneal neovascularization growth , animals were sacrificed 16 days after the alkali burn , take the the corneal biopsy observation of structural changes , immunohistochemical detection of VEGF protein , transmission electron microscopy to study the ultrastructural changes of the cornea PCR detection corneal tissue VEGFmRNA of expression levels . Results : C , D group rat corneal neovascularization after alkali burn each time point area were less than group B , group C , group D and group B neovascularization area of ??difference was statistically significant ( P lt ; 0.05 ) ; D group compared with C group angiogenesis inhibition is a little stronger , the difference was not statistically significant ( P gt ; 0.05 ) 16 days after corneal alkali burn Immunohistochemical examination showed no statistically significant difference ( P GT Group C and Group D VEGF protein ; 0.05 ) , the rest of the group pairwise comparison of VEGF protein difference was statistically significant . TEM examination showed the first 16 days after alkali burn , A group of normal cornea layers of cells close , normal structure . C, D treatment group than in group B structure changed little . PCR results showed corneal alkali burn day 16 C , the relative expression level of D group VEGFmRNA the less than group B , but higher than the A group . Conclusion: The point of the eye and subconjunctival injection of recombinant human endostatin ( ex degrees) that can effectively inhibit corneal neovascularization induced by alkali burn .

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