Flap-free Epi-LASIK and Epi-LASIK two surgical postoperative corneal haze (Haze) the development of the situation and to explore its mechanis" />
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Comparative Study to the Haze Forming Experiment after Epi-LASIK and Flap-free Epi-LASIK Operation to Rabbits

Author: XiangYi
Tutor: DuZhiYu
School: Chongqing Medical University
Course: Ophthalmology
Keywords: Flap-free Epi-LASIK Epi-LASIK Haze
CLC: R779.63
Type: Master's thesis
Year: 2008
Downloads: 43
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Abstract


Objective: To observe and compare Epi-LASIK' dissertation">Flap-free Epi-LASIK and Epi-LASIK two surgical postoperative corneal haze (Haze) the development of the situation and to explore its mechanism. Methods: 21 healthy New Zealand white rabbits, 18 rabbits a line the other eye line Flap-free Epi-LASIK Epi-LASIK-10D cutting VISX20/20 excimer laser for the other three rabbits as a blank control . Each group after the operation in January, February, March 3 points on a regular basis in the slit lamp Haze grading and assessment. 6 animals in each group were randomly selected eye, corneal tissue after take three equal portions: half of the slices for histological examination, and the other half frozen for molecular biology. Part I: Take corneal tissue using transmission electron microscopy, reverse transcriptase polymerase chain reaction (reverse transcription-polymerase chain reaction, RT-PCR) and immunohistochemistry (immunohistochemistry, IHC) method to observe Haze relevant indicators. Part II: transforming growth factor (TGF-β2) and alkaline the fiber cell factor (b-FGF) expression levels, compare and explore its two surgical postoperative Haze formation and development process using immunohistochemical methods to detect The role. Results: The patients at each time point Haze grading levels of α-smooth muscle actin (α-SMA) and type III collagen mRNA expression results Flap-free Epi-LASIK and Epi-LASIK postoperative each time point difference between the groups There was no statistically significant (P gt; 0.05). Transmission electron microscopy test results found that each group a different point in time on the basic integrity of cortical structure and basement membrane, January and March 2 experimental group compared with the normal stromal cells, collagen relatively neatly arranged; 2 stromal cells of the two experimental groups was active, collagen derangement. RT-PCR analysis: Epi-LASIK group and Flap-free Epi-LASIK group mRNA expression of collagen type III postoperative time point, there was no statistically significant difference in the dynamic expression; the postoperative type III collagen fibers mRNA group showing a first characteristics, and then decreased after 2 months and reached the peak. Immunohistochemical detection: Epi-LASIK group and Flap-free Epi-LASIK group of postoperative time points of TGF-β2, b-FGF expression differences without statistical significance (P gt; 0.05); expression levels of TGF-β2 The comparison showed a gradual decline in the group characteristics; b-FGF expression levels in the two experimental groups showed no significant difference (P gt; 0.05), and were extended over time continues to rise trend. Conclusion: Rabbit Flap-free Epi-LASIK and Epi-LASIK postoperative Haze formation and a similar level of development; The two surgical postoperative affect Haze formation and development of key regulatory factor TGF-β2 and b-FGF was no significant difference.

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CLC: > Medicine, health > Ophthalmology > Eye surgery and surgery > Retinal photocoagulation and laser eye surgery
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