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The Effects of Transactivator of HCV NS5A-NS5ATP9 Gene on the Proliferation of Hepatoma Cells

Author: ZuoJi
Tutor: ZhengXiBang；ChengJun
School: Guangxi University
Course: Clinical Veterinary Medicine
Keywords: NS5ATP9 gene Real-time PCR Cell Cycle Cells
CLC: R735.7
Type: Master's thesis
Year: 2008
Downloads: 41
Quote: 0
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Abstract


Hepatitis C virus (HCV) is a single strand RNA virus genome size of approximately 9.4kb. HCV infection causes chronic hepatitis, cirrhosis and even liver cancer . HCV genome is only one open reading frame encoding a 3010-3033 amino acid polyprotein by cellular and viral proteases cut , form structural proteins and non-structural proteins. In which the non- structural proteins of HCV NS5A play an important role in pathogenesis . NS5A gene is located between 6258-7601 nt , encoding a molecular weight of 56 kD of NS5A protein in the cytoplasm . NS5A protein with a variety of biological functions , in HCV polyprotein maturation and replication of RNA ; as a transcriptional activating factor affecting cell signal transduction pathway , activate a variety of viruses and cells, promoters, control cells , viral gene expression regulate cell growth, apoptosis , and immunity , and cells infected with HCV is closely related to the process of malignant transformation . NS5ATP9 is HCV NS5A trans-activation of the target protein, which with apoptosis and proliferation are closely related to the development of liver fibrosis . To investigate the HCV NS5A intracellular NS5ATP9 expression and expression of exogenous NS5ATP9 hepatocyte proliferation and cell cycle effects , the study of molecular biology techniques , namely the recombinant plasmid pcDNA3.1, pcDNA3.1-NS5A and transfected with pcDNA3.1-NS5ATP9 HuH-7 and HepG2 cells by G418 selection , stable transfected cell clones . Using real-time quantitative PCR NS5ATP9 mRNA expression showed that , compared with control group transfected with pcDNA3.1-NS5A hepatocytes , NS5ATP9 mRNA expression was significantly increased ; transfected with pcDNA3.1-NS5ATP9 found in liver cells by flow cytometry to detect cell cycle found in the number of S-phase cells increased significantly by chemiluminescence found in living cells significantly promote hepatocyte proliferation . The results for further research and NS5ATP9 NS5A interaction and its effect on cell biology of liver cell basis .

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
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