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The Expression of Skp2 and P27 in Oral Verrucous Carcinoma

Author: WangRenQin
Tutor: TangZhanGui
School: Central South University
Course: Clinical Stomatology
Keywords: Oral cancer Oral squamous cell carcinoma Skp2 p27 Cell cycle Immunohistochemistry
CLC: R739.8
Type: Master's thesis
Year: 2008
Downloads: 36
Quote: 0
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Abstract


Objective: To study the S phase kinase related protein Skp2 factors and cell cycle inhibitor p27 expression in the OVC characteristics, and to explore its relationship with clinical and pathological features, new oral verrucous cancer diagnosis, treatment and prognosis ideas, in order to further understand the the OVC with oral squamous cell carcinoma difference and contact reference. Methods: Immunohistochemical SP method detected 15 cases of oral verrucous carcinoma, 30 patients with oral squamous cell carcinoma and 15 cases of normal oral mucosa specimens Skp2 and p27 protein expression levels, SPSS12.0 software was used for statistical analysis. In: 1.Skp2 expression of Skp2 in OVC group, the expression in squamous cell carcinoma group was significantly higher than the normal group (P <0.05), verrucous carcinoma and squamous cell carcinoma group showed no significant difference (P> 0.05) . Verrucous carcinoma group, grouped by age, gender, tumor site and clinical stage Skp2 expression had no significant difference (P> 0.05); clinical sub-type grouping, Skp2 expression was significantly different (P <0.05) . Squamous cell carcinoma, grouped according to age, sex, tumor location, Skp2 expression had no significant difference (P> 0.05), grouped by clinical stage and degree of malignancy, the higher the clinical stage, the lower degree of differentiation, lymph node metastasis, Skp2 expression is higher, there is a significant difference (P <0.05). 2.p27 the expression of p27 in Oral carcinoma, squamous cell carcinoma, and the normal group, the positive expression rate of the three groups were 43.3%, 53.3% and 93.3%, three groups overall more significant difference (P <0.05) . Oral carcinoma, squamous cell carcinoma group was significantly lower than that of the normal group (P <0.05), verrucous carcinoma and squamous cell carcinoma between the two groups showed no significant difference (P> 0.05). The verrucous carcinoma group, grouped according to age, gender, tumor site and clinical stage, p27 expression was no significant difference (P> 0.05); clinical grouping, infiltration / cyst type p27 expression was significantly lower than the outer wild type; squamous cell carcinoma, grouped by age, sex, tumor location, clinical stage, expression of p27 had no significant difference (P> 0.05), grouped by degree of malignancy, the lower the degree of differentiation, lymph node metastasis , p27 expression is lower, the difference was significant (P <0.05). 3.Spearman rank correlation analysis showed that Oral cancer, oral squamous cell carcinoma of Skp2 and p27 expression showed a significant negative correlation (r 1 = -0.935, P <0.05), (r 2 = -0.639, P <0.05). Conclusion: 1.Skp2 and p27 may be involved in the OVC and oral squamous cell carcinoma, the development process, and is closely related to the degree of malignancy. 2.Skp2 and p27 may work from the Oral cancer and oral squamous cell carcinoma invasion and metastasis. 3.Skp2 important factor may be caused by malignant tumors, p27 downregulation.

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