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iNOS inhibitor of nasopharyngeal carcinoma CNE-2 cells explore the role of

Author: ZhangHaiXia
Tutor: HuChunHong
School: Central South University
Course: Oncology
Keywords: iNOS inhibitor SMT Cisplatin Nasopharyngeal Apoptosis Chemosensitizing
CLC: R739.63
Type: Master's thesis
Year: 2008
Downloads: 61
Quote: 0
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Abstract


Objective : To observe the inducible nitric oxide synthase (iNOS) inhibitor SMT whether nasopharyngeal carcinoma cell line CNE-2 inhibition of cell proliferation and apoptosis ability to explore SMT resistance to cisplatin effect of CNE-2 cells whether there sensitizing effect and molecular mechanism for the treatment of advanced nasopharyngeal carcinoma fumble rational drug combinations provide experimental evidence Methods: a selection of nasopharyngeal carcinoma cell line CNE-2 as the research object, A549 as a control , RT-PCR test whether CNE-2 expression iNOS. 2 with different concentrations of SMT, cisplatin , the two combined effect on CNE-2 cells , the intervention CNE-2 cells , MTT test observe its effect on cell viability . 3 Hoechst33258 staining of apoptotic morphological changes . 4 Flow cytometry (FCM) to detect cell apoptosis index and apoptosis levels. 5 nitrate reductase method to measure the content of NO . Results: 1 to high expression of iNOS in lung cancer cell line A549 as a positive control , CNE-2 cells confirmed iNOS expression . 2.SMT concentration-dependent manner effectively suppressed CNE-2 cells. 3.0.5umol/mL of SMT and 6μg/ml cisplatin common intervention CNE-2 cells , compared with cisplatin alone SMT or CNE-2 inhibition of apoptosis obvious morphological changes , apoptotic bodies and nuclear condensation phenomenon increased. 4.0.5umol/mL of SMT and 6μg/ml cisplatin common intervention CNE-2 cells , compared with cisplatin alone CNE-2 cells inhibited apoptosis induced apoptosis rate was (22.26 ± 1.37)% and (38.8 ± 1.25)%, P value <0.05. 5.0.5umol/mL of SMT and 6μg/ml cisplatin common intervention CNE-2 cells , compared with the normal group , the amount of NO in the two groups were (9.05 ± 0.02 ) umol / lVS (14.79 ± 0.03) umol / l, there is statistically significant. Conclusion : 1.SMT effectively in a concentration -dependent inhibition of CNE-2 cells. 2.SMT resistant to cisplatin effect of CNE-2 cells with chemotherapy sensitizing effect , which may be related to the amount of NO , but the exact mechanism remains to be sensitized further study.

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CLC: > Medicine, health > Oncology > Department of Otolaryngology tumor > Pharyngeal tumors
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