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Study on Effect of Celecoxib on CNE-2Z Cell Lines in Human Nasopharyngeal Carcinoma

Author: XiangYinZhou
Tutor: WeiLianZhi
School: Chongqing Medical University
Course: Otorhinolaryngology
Keywords: Celecoxib Cyclooxygenase-2 Nasopharyngeal carcinoma cell line CNE-2Z Growth inhibition PCNA Caspase-3
CLC: R739.63
Type: Master's thesis
Year: 2008
Downloads: 39
Quote: 0
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Abstract


Objective: To study the COX-2 expression of human nasopharyngeal carcinoma cell line CNE-2Z and study its inhibitor celecoxib on nasopharyngeal carcinoma cell line CNE-2Z growth inhibition and possible mechanisms . Explore the COX-2 inhibitors in human nasopharyngeal value in the prevention and treatment of the feasibility and theoretical basis for future clinical studies . : Human nasopharyngeal carcinoma cell line CNE-2Z for the study, in vitro drug sensitivity test (MTT) observed different concentrations and duration of action of celecoxib on cell growth inhibition ; Immunocytochemistry detection of proliferating cell nuclear antigen (PCNA) and cyclooxygenase -2 (COX-2) expression ; flow cytometry (FCM) detection of drug action , the cell cycle distribution ; Western-blot detection of celecoxib on the expression of caspase-3 . Results : (1) MTT celecoxib on nasopharyngeal carcinoma cells CNE-2Z growth inhibition , and showing a time - and dose - dependent manner ; increase with the concentration of the drug and the role of time , the number of viable cells gradually reduce ; ( 2) plug celecoxib proliferation of CNE-2Z cells inhibit drug effects after 48h of PCNA expression was significantly reduced ; ( 3 ) CNE -2Z cells in the presence of COX-2 expression and with celecoxib cloth concentrations increased and decreased expression ; (4 ) the flow cytometry results showed that celecoxib can cause CNE-2Z cells in G0/G1 phase arrest ; ( 5 ) Western- Blot results show that , with the celecoxib concentration increased caspase -3 protein expression gradually increased . Conclusion : 1 . COX-2 protein expression in the human nasopharyngeal carcinoma cell line CNE-2Z cells . Celecoxib celecoxib by inhibition of cell growth and proliferation , so that the cell cycle arrest in G0/G1 phase and inhibit the growth of human nasopharyngeal carcinoma cell CNE-2Z . 3 plug celecoxib inhibition of human nasopharyngeal carcinoma cells CNE-2Z growth mechanism and COX-2, PCNA and Caspase-3 -related . Celecoxib celecoxib for nasopharyngeal cancer prevention research has important significance , COX -2 inhibitors is expected to play an important role in the prevention and treatment of human nasopharyngeal carcinoma .

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CLC: > Medicine, health > Oncology > Department of Otolaryngology tumor > Pharyngeal tumors
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