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Study on the Effects of NDGA, a General Lipoxygenase Inhibitor, on the Proliferation and Apoptois in Prostate Cancer Cell PC-3

Author: TanHuaKai
Tutor: ZhuShaoXing
School: Fujian Medical
Course: Surgery
Keywords: NDGA Prostate cancer Apoptosis
CLC: R737.25
Type: Master's thesis
Year: 2008
Downloads: 41
Quote: 0
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Abstract


Objective: To investigate the lipoxygenase inhibitor NDGA prostate cancer PC-3 proliferation and apoptosis and its mechanisms , and hope for a new drug to treat prostate cancer . METHODS: Cultured prostate cancer PC - 3 , to different concentrations ( 0 μmol · L -1 < / sup> 20μmol · L -1 < / sup> 40μmol · L -1 < / sup> 80μmol · L -1 160 μmol · L -1 ) the NDGA intervention cell by cell morphological observation and MTT assay by TUNEL assay NDGA on PC-3 prostate cancer cells proliferation and induction of apoptosis , the activation of Caspase-3 expression was detected by flow cytometry . RESULTS : MTT method and Tunnel assay 0μmol · L -1 and 20μmol L -1 < / sup> NDGA on the growth of prostate cancer PC-3 significantly inhibit the proliferation and induction of apoptosis ( P gt; 0.05) , 40μmol · L -1 80μmol · L -1 < / sup > and 160 μmol · L -1 < / sup > prostate cancer PC-3 significant inhibition of proliferation and induction of apoptosis (P lt; 0.05); flow cytometry to detect the activation of Caspase-3 expression 40μmol · L -1 80μmol · L -1 < / sup> and 160 μmol · L -1 NDGA intervention group activated Caspase-3 expression was significantly higher (P lt; 0.05), 0μmol · L -1 and 20μmol L -1 < / sup > the NDGA intervention group without significantly higher (P gt; 0.05). Conclusion : NDGA can inhibit prostate cancer cell line PC-3 proliferation and induced apoptosis in a dose - and time - dependent manner , the mechanism may be through the induction of prostate cancer PC-3 Caspase-3 expression activity enhancement , ultimately leading to apoptosis .

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Male genitalia tumors > Prostate cancer
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