Dissertation > Excellent graduate degree dissertation topics show

The Study on the Action of p38-HSP27 Signaling Pathway Mediating the Actin Cytoskeleton Remodeling in Renal Tubular Epithelial Cells during ATP Depletion

Author: ZhangLiJia
Tutor: GuLuo
School: Nanjing Medical University
Course: Physiology
Keywords: ATP depletion Tubular epithelial cells Actin cytoskeleton p38 kinase Heat shock protein 27 (HSP27)
CLC: R692.6
Type: Master's thesis
Year: 2008
Downloads: 103
Quote: 0
Read: Download Dissertation

Abstract


Previous studies confirmed ischemic renal injury, tubular epithelial cell actin cytoskeleton remodeling is impaired renal function worsening ischemic renal injury, specific mechanisms of actin cytoskeleton remodeling unclear. Renal ischemic state analog of this study, in vitro ATP depletion model based on study ATP depletion renal tubular epithelial cell actin cytoskeleton reconstruction of the situation and the actin cytoskeleton remodeling the possible mechanisms to a period of The pathogenesis of ischemic renal injury provides a theoretical basis. According to the results of previous studies, we speculate that the p38-HSP27 signaling pathway may be involved in ATP depletion of renal tubular epithelial cell actin cytoskeleton remodeling process. In this study the rat renal proximal tubular epithelial cell line (NRK52E cells), after fluorescent staining ATP missing actin cytoskeleton reconstruction; cells by immunoblotting p38 kinase expression and activity changes , simultaneous analysis of HSP27 expression, the change of the level of phosphorylation of its intracellular localization. F-actin protein staining results showed that ATP deletion NRK52E cell actin cytoskeleton significantly reconstructed F-actin protein content was significantly higher, and the processing time related to ATP depletion. This result indicates that the ATP depletion actin cytoskeleton occurs polymerized reconstructed changes. Western blot results showed that the ATP deletion of p38 kinase activated quickly, HSP27 phosphorylation was significantly strengthened, and HSP27 redistribution phenomenon from the skeleton components to the cytoplasmic constituents. After the use of the p38 kinase inhibitor SB203580 pretreatment of the cells of p38 kinase activity was able to significantly inhibit the phosphorylation of HSP27 of significant inhibition the heavy of HSP27 distribution phenomenon can be reversed; while ATP depletion due to the actin cytoskeleton constitutive phenomenon can be partially reversed by ATP missing due to F-actin content increased significantly inhibited. The results of this study show that the p38 kinase through the regulation of HSP27 phosphorylation state affects its weight distribution, and then participate in renal tubular epithelial cells in the reconstruction of the actin cytoskeleton partially mediated ATP depletion. Through the above research, we draw the following conclusions: 1.ATP missing tubular epithelial cell actin cytoskeleton polymerization occurs reconfigurable change. 2.p38-HSP27 signaling pathways involved in part mediated ATP depletion of renal tubular epithelial cell actin cytoskeleton reconstruction effect.

Related Dissertations

  1. The Roles and Mechanism of HIF-2α in Pathogenesis of Ischemic/Hypoxic Acute Kidney Injury,R692
  2. Lipoxin A_4 on CTGF and TGF-β_1 induced human renal tubular epithelial cells of,R692
  3. Research on the Role of γ-GCS in the Interstitial Fibrosis of Lupus Nephritis,R593.242
  4. Effect of RHuEPO Pretreatment on PI3K/Akt/GSK-3β Signaling Pathway in Human Renal Tubular Epithelial Cells Apoptosis Induced by Ischemia-reperfusion Injury,R363
  5. Study on Cellular and Molecular Mechanisms of Brain and Kidney Injury Induced by Lead Acetate,R114
  6. Studies on Cell Microfilaments and Volatile Metabolies of Rice Inoculated with Magnaporthe Grisea,S435.111.41
  7. Effect of Rosiglitazone on the Apoptosis of Cultuerd NRK-52E Cells Induced by CsA,R96
  8. The Effect of EGB761 on Renal Tubular Epithelial Cells Apoptosis Induced by Hydrogen Peroxide Invitro,R285.5
  9. Study on Effects of Lipopolysaccharide on the Transcriptional Activity of GRP78 and CHOP in Rat Renal Tubular Epithelial Cells,R692
  10. Study on Culture and Immortalization of Renal Tubular Epithelial Cells in Newborn Goat,R692
  11. Effects of NF-κB P65 Antisense Oligodeoxynucleotide on the Expression of Extracellular Matrix Induced by High Glucose in Proximal Tublar Epithelial Cells,R587.2
  12. The Effect of Rho/ROCK Signal Transduction Pathway on F-actin Cytoskeleton in Human Tongue Squamous Cell Carcinoma,R739.8
  13. The Cytotoxicity of Iodinated Contrast Media on Human Renal Tubular Epithelial Cell,R692
  14. Role and Mechanism of Peripheral-type Benzodiazepine Receptor in Human Renal Tubular Cells Injury Induced by Postasphyxial-Serum of Neonates,R722.1
  15. Protective Effects of Recombinant Human Erythropoietin on Apoptosis of Human Renal Tubular Cells (HK-2) during Morphological Changes of Mitochondria Induced by Postasphyxial-serum in Neonates with Asphyxia,R722.1
  16. Effect of Erythropoietin and Astragalus on Expression of TLR4/MyD88 in Human Renal Tubular Cells Induced by Postasphyxial-Serum of Neonates,R722.12
  17. Rosmarinic acid inhibition of renal tubular epithelial - mesenchymal transdifferentiation role and mechanism of,R692
  18. Angiotensin - ( 1-7 ) on angiotensin Ⅱ-induced renal tubular epithelial cells of,R692
  19. Protective Effect of BMP-7 Against Aristolochic Acid-induced Renal Tubular Epithelial Cell Injury,R692.9
  20. The Effects of Enhanced Uv-b and He-ne Laser on Microfilament Cytoskeleton in Wheat,S512.1
  21. The Expression of Respones Gene to Complement-32 in Renal Tissue on Children with IgA Nephropathy,R726.9

CLC: > Medicine, health > Surgery > Urology ( urinary and reproductive system diseases) > Kidney disease > Renal tubular,glomerular disease
© 2012 www.DissertationTopic.Net  Mobile