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Objective: To investigate acute spinal cord injury cyclosporine A (CsA) on the expression of iNOS and apoptosis. Methods: Rat animal model of acute spinal cord injury. 108 rats were randomly divided into simple laminectomy group (control group), spinal cord injury group (injury group), spinal cord injury after CsA treatment group (treatment group). Were sacrificed at different time points of anesthesia, intubation, spinal cord injury coverage, fixed overnight, embedded, sliced. HE staining of spinal cord injury with pathological changes; using immunohistochemical EnVision iNOS expression was detected in situ end labeling (TUNEL method) labeled cells; utilize HPIAS-2000 high-resolution color graphic report management system pathology iNOS Quantitative analysis of the expression, apoptosis index under a microscope to detect apoptosis. Results: 1, HE staining under light microscope: Visible control rats spinal cord white matter, gray matter and gray matter in the anterior horn and posterior horn and the central tube. Injury and treatment groups 6h after spinal cord injury has been bleeding, no necrosis. With time, the spinal cord injury group showed a broad focal hemorrhage and bleeding after the formation of cysts, neuronal degeneration and necrosis, dissolution, there are a large number of small glial cell proliferation and neutrophil infiltration. With prolonged treatment with focal hemorrhage spinal cord, neuronal cell swelling, some nerve cell degeneration and necrosis, but bleeding, microglial proliferation and inflammatory cell infiltration injury group than light. 2, iNOS in the control group showed a suspicious expression spinal cord, spinal cord injury in rats showed a strong expression, the treatment group showed a weak expression spinal cord. 6h injury after injury and treatment group that is the expression of iNOS; injury 7d iNOS reached the peak, 10d expression was significantly decreased. INOS expression in the treatment group were lower than the injury, the peak occurs after injury 3d. Three groups at each time point of the image analysis results by single-factor analysis of variance, the difference was statistically significant (6h: F = 89. 449, P lt; 0.01; 12h: F = 24.640, P lt; 0.01; 24h: F = 72.699 , P lt; 0.01; 3d: F = 190.527, P lt; 0.01; 7d: F = 160.347, P lt; 0.01; 10d: F = 328.885, P lt; 0.01). 3, the control group scattered spinal cord, a small amount of TUNEL-positive cells labeled, TUNEL labeling injury group showed a lot of positive cells, the treatment group showed moderate amount of TUNEL-positive cells labeled. 6h injury after injury after injury CsA-treated group and the group that is labeled TUNEL-positive cells; injury 7dTUNEL marker-positive cells reached the peak after injury 10dTUNEL labeled positive cells were significantly reduced. TUNEL-positive cells in the treatment group compared with injury group labeled low, the peak occurs after injury 3d, then gradually decreased. Three groups at each time point apoptosis index results Univariate analysis of variance, the difference was statistically significant (6h: F = 180.432, P lt; 0.01; 12h: F = 491.523, P lt; 0.01; 24h: F = 594.093, P lt; 0.01; 3d: F = 1110.324, P lt; 0.01; 7d: F = 837.660, P lt; 0.01; 10d: F = 389.124, P lt; 0.01). Conclusion: CsA inhibited after acute spinal cord injury in rats iNOS expression and apoptosis, reduce spinal cord injury.
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