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Methylation Pattern of BCSG1 Gene and Its Relationship with Gene Expression in Breast Tumor

Author: TanJianXin
Tutor: SunYuJie
School: Nanjing Medical University
Course: Cell Biology
Keywords: BCSG1 gene CpG island methylation Gene expression Breast cancer Racial differences
CLC: R737.9
Type: Master's thesis
Year: 2008
Downloads: 78
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Abstract


The BCSG1 gene was discovered in 1997 breast cancer -specific genes . Foreign study found that close to high BCSG1 gene expression and breast cancer , abnormal BCSG1 gene exon region CpG island demethylation , one of the main reasons leading to abnormally high expression in breast cancer . In order to further understanding of breast cancer patients BCSG1 gene methylation level change and its relationship with gene expression asked , to explore BCSG1 gene methylation potential as a breast cancer molecular markers , methylation-specific PCR ( MSP ) method and DNA sequence analysis, were detected 117 cases of paraffin-embedded breast cancer tissues , 20 pairs of liquid nitrogen storage of fresh breast cancer and adjacent tissues and 22 cases of benign breast lesions gene exons BCSG1 the CpG island methylation status and real -time PCR detection of the corresponding fresh breast cancer , cancer tissue and benign breast lesions BCSG1 mRNA expression level . The results showed that : (1 ) breast cancer tissue and benign breast lesions of BCSG1 gene exon regions of CpG island methylation status was no significant difference , there are unmethylated , of which 50 % to 60 % of organizations in the methylated and unmethylated state coexist , and the methylation status of genes with various clinical parameters were not correlated ; (2 ) in breast cancer , BCSG1 gene mRNA expression its methylation state-related , and in adjacent benign lesions , the methylation status of mRNA expression ; ( 3 ) breast cancer the adjacent tissues in BCSG1 gene mRNA expression level of five times in the cancer tissue , benign lesions the organization no significant Majesty difference . Our results show that in the female breast tissue , the area of BCSG1 gene exon CpG island methylation status of its unique pattern of methylation associated with BCSG1 gene expression by the breast tissue pathology the impact of the state , BCSG1 gene methylation status and transcriptional regulation mechanism with a high degree of heterogeneity , and may be related to genetic background and ethnic differences , the Chinese population BCSG1 gene and breast cancer relationship has yet to be further identified .

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Breast tumor
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