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Objective To investigate the impact of recombinant human growth hormone on trauma rat protein synthesis and intestinal mucosa structure. The method completely randomized controlled study, 24 adult healthy Wistar rats of either sex ,270-290 grams, by the Hunan Agricultural University Animal Center. All rats were fed for a week, single cage feeding. Trauma rat model were randomly divided into two groups, the control group were fed with milk for early enteral nutrition (EEN) saline subcutaneously, the experimental group of early enteral nutrition combined with recombinant human growth hormone (rhGH) with milk filling Hey subcutaneous injection of recombinant human growth hormone. The control group and the experimental group at each time point, 4, and 7 days after injury from each of four rats were anesthetized weighing blood were killed. Serum total protein, serum albumin and serum prealbumin content, at the same time to take part of the ileum intestine morphological observation and intestinal mucosa interleukin-1 (IL-1) detection. (1) both subjects pre-injury results in body weight, serum total protein, albumin, prealbumin and other indicators was no significant difference (P> 0.05). ② after injury, weight comparison: the first day after the injury, body weight of the experimental group and control group, the difference was not statistically significant (P> 0.05), the fourth day after injury, the experimental group weight was higher, the difference was statistically significant (P <0.05), the real injury after seven days, the experimental group weight higher than that of the control group, the difference was statistically significant (P <0.05), serum total protein content (3) injury comparison: serum total protein after injury each time point comparison between the control group and the experimental group were not statistically significant (P> 0.05), serum albumin level comparison: ④ after injury after injury, serum albumin comparison between the control group and the experimental group at each time point were not statistically significant (P> 0.05). ⑤ after injury, serum prealbumin content: 1, the experimental group and control group, serum albumin, the difference was not statistically significant (P> 0.05), the fourth day after injury, levels of serum before albumin levels higher than those in the control group, the difference was statistically significant (P <0.05), the first 7 days after injury, levels of serum prealbumin level was higher, the difference was statistically significant (P <0.05), ⑥ intestinal mucosal morphology: the first day after injury, the control group and the experimental group were seen the end of the villi of the intestinal mucosa epithelial cell necrosis, villous edema, inflammatory cell infiltration. 4 days after injury, the control group compared with the first day of necrosis reduced visible inflammatory cell infiltration, villus thickening edema, height reduced, while the experimental group visible inflammatory cell infiltration, inflammatory response than the control group to reduce and fluff height is greater than the level of the control group. The 7th day after the injury, villus height of the control group compared with the first four days of growth, still visible edema, inflammatory cell infiltration, the structural integrity of the experimental group fluff, no edema. ⑦ mucosa IL-1: the rats intestinal mucosa IL-1 expression levels were elevated, one day after the injury is the most obvious, after a gradual downward trend over time. The first day after injury, there is no significant difference (P> 0.05) between the control group and the experimental group. 4 days after injury, two groups of IL-1 expression compared to day 1 were decreased in the experimental group was more evident in the control group is higher than the level of the experimental group (P <0.05). The first 7 days after injury, two groups of IL-1 expression than the 4th day decline further, more obvious decline in the experimental group, significantly lower than the level of the control group (P <0.05). Conclusion Early enteral nutrition combined with recombinant human growth hormone in promoting severe trauma in rats protein synthesis, to maintain the integrity of the intestinal mucosa structure, promote hair growth, reduce inflammation better than a simple enteral nutrition.
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