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Objective: To study Steady magnetic field on hypertrophic scar fibroblasts in vitro cell proliferation , TGF-β1 and collagen Ⅰ expression intensity of inhibition ; Steady different intensities observed magnetic field on hypertrophic scar fibroblasts in vitro cell proliferation , TGF-β1 and collagen Ⅰ expression of strength ; explore steady magnetic mechanism of anti- hypertrophic scars . Methods: vitro cultured skin hypertrophic scar fibroblasts in vitro 5-6 generations, is divided into four experimental groups , Group A 0T ( control group ), B group 0.1T, C group 0.2 T, D group 0.3T; the four experimental groups without treatment factors first cultured for 12 hours, then in the B group, C group , D group , respectively, with strength of 0.1T, 0.2T, 0.3T magnetic thermostatic control group A without a magnetic field , and then cultured for 12 hours, 24 hours , 60 hours , at four time points by cell counting and Western Blot and other methods to detect the scar fibroblast cell number , TGF-β1 and collagen Ⅰ expression on the number of total gray values. Analysis of Steady magnetic field on scar fibroblasts TGF-β1 and collagen Ⅰ expression. Results: The treatment group (B group , C group , D group ) count fibroblasts compared with the control group (A ) decreases and TGF-β1 and collagen Ⅰ expression also significantly reduced compared with the control group (P <0.05 ) ; between treatment groups , with the magnetic intensity , the number of skin scar fibroblasts gradually decreased , TGF-β1 and collagen Ⅰ expression intensity gradually weakened . Conclusion: Steady magnetic field inhibits proliferative scar fibroblast proliferation , decreased TGF-β1 and collagen Ⅰ expression .
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