Dissertation > Excellent graduate degree dissertation topics show

The Mechanism of Apoptosis Induced by Paclitaxel Combined with TRAIL in MCF-7 Breast Cancer Cell Lines

Author: ZhangChuanTao
Tutor: ZuoZhongSheng；ShiYeHui
School: Tianjin Medical University
Course: Oncology
Keywords: Breast tumor Taxol TRAIL Apoptosis Comprehensive treatment
CLC: R737.9
Type: Master's thesis
Year: 2008
Downloads: 166
Quote: 0
Read: Download Dissertation

Abstract


Objective: To explore the paclitaxel-induced tumor necrosis factor-related apoptosis-inducing ligand (tumor necrosisfactor-related apoptosis inducing ligand, TRAIL) MCF-7 breast cancer cell lines apoptosis and its mechanism. Methods: MTT was detected concentration of single-agent paclitaxel, a single agent TRAIL and paclitaxel with TRAIL different joint administration program inhibitory effect on the growth of MCF-7 cells; the Annexin V-FITC/PI staining flow cytometry analysis of different co-administration the impact of the program on apoptosis in MCF-7 cells; PI staining flow cytometry analysis of the impact of different dosing regimens of MCF-7 cell cycle distribution; RT-PCR assay TRAIL receptors DR4 and DR5 in MCF-7 breast cancer The expression level in the cells. RESULTS: MTT method found paclitaxel in a dose-dependent inhibition of MCF-7 breast cancer gradually increased with the concentration of the drug increased the inhibition rate IC50 for 4.85μM; of TRAIL concentration <0.05μM dosing groups with blank group inhibition difference was not statistically significant (P> 0.05), when drugs ≥ 0.50μM dosing group and blank group inhibition of the difference was statistically significant (P <0.05), but gradually increased with the concentration of the drug suppression role not seen a corresponding increase (P> 0.05), show a certain resistance; election paclitaxel 0.5μM, TRAIL 0.5μM concentration of co-administered. MTT showed that the different co-administered programs inhibition of MCF-7 breast cancer cell lines of different strength, joint administration group 1 (administration of paclitaxel and TRAIL) and co-administered group 2 (the first giving TRAIL paclitaxel) synergy: the co-administered group 3 (paclitaxel first be given TRAIL) visible synergies, select group of the co-administered dosing regimen for flow cytometry. Double staining of Annexin V-FITC/PI found paclitaxel combined with TRAIL compared with the single-agent group can induce apoptosis in MCF-7 breast cancer cells; blank control group, paclitaxel (0.5μM × 48h) group, TRAIL (0.5μM × 24h) group, co-administration (paclitaxel TRAIL continue role 24h 24h after the end of the two drugs concentration apoptosis rate were 0.50μM) group (7.56 ± 2.23)% and (22.6 ± 2.44)%, respectively ( 12.36 ± 0.47)%, (61.56 ± 5.52)%. PI assay, TRAIL group compared with blank control group, cell cycle distribution difference was not statistically significant (P> 0.05), TRAIL does not affect the cell cycle distribution; paclitaxel administered in combination with TRAIL group with paclitaxel group there was no difference of the cell cycle significance (P> 0.05). RT-PCR confirmed at the mRNA level, paclitaxel can induce DR5 expression level of MCF-7 breast cancer cells increase DR4 expression levels had no significant effect. Conclusion: MCF-7 breast cancer cell lines are sensitive to paclitaxel, but poor sensitivity to TRAIL; paclitaxel combined with TRAIL visible synergies; induced apoptosis synergistic inhibition of tumor cell; TRAIL no obvious role in cell cycle arrest, and not impact the role of paclitaxel on cell cycle arrest; collaborative mechanism may be related with paclitaxel induction of DR5 upregulation. Paclitaxel administered in combination with TRAIL way to provide a theoretical basis for the next step in vivo animal experiments.

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  4. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  5. The Roles of Microtubules and Protein Phosphatase AtPP2CA in Regulation of Stomatal Movement in Arabidopsis Thaliana,Q945
  6. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  7. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  8. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  9. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  10. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  11. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  12. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  13. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  14. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  15. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  16. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  17. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  18. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  19. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  20. The Signal Mechanism Underlying DON Induced Apoptosis in BHK-21 Cells,S856.9
  21. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3

CLC: > Medicine, health > Oncology > Genitourinary tumors > Breast tumor
© 2012 www.DissertationTopic.Net  Mobile