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Objective: Patients with acute cerebral contusion in neurosurgery is very common primary brain contusion generally do not need surgical treatment, but when the secondary damage caused by intracranial hypertension even herniation, surgery necessary. Surgery in the management of patients choose to have cerebral protection of narcotic drugs is necessary propofol as a clinical model narcotic drugs, can reduce cerebral metabolic rate, reduced cerebral blood flow, reduce intracranial pressure has a protective effect on neurons gradually become a research hot spots. C-fos protein is an immediate early gene is linked cell biochemical changes in cells eventually specifically react to stimuli intermediary, its expression and its expression as a the molecular biology flag determine the extent of damage to nerve cells. C-fos gene expression and apoptosis is proportional to the degree of, intraoperative application with cerebral protective effects of anesthetic drugs, effectively reducing the number of c-fos protein expression on postoperative recovery and reduce mortality has a positive significance. Less propofol expression of c-fos protein in brain contusion, the experimental observation of propofol on cerebral contusion hippocampal tissue around the c-fos gene expression, protein levels on brain c-fos protein expression after contusion. Methods: Wistar rats were male. Weight (241.6 ± 10.5) were randomly divided into sham operation group (A), after brain injury in the control group (B), and brain contusion after propofol treatment group (C), C group propofol phenol dosage of 60 mg / The kg.h uniform pumped. Using a rat model of cerebral contusion. Distribution of positive cells was observed under the brain contusion 120min, the decapitated brain, take whole brain embedded in paraffin, sliced ??(slice thickness 5 um), application of c-fos gene immunohistochemistry, low-power microscope (× 10) situation, was observed under high magnification (× 20) differentiation Fos staining positive cells and the density and gradation value as the number of positive cells. positive cells detected in the brain of c-fos protein expression level of the surrounding tissue in the hippocampus. Results: cerebral contusion cortex, hippocampus, and the hippocampus edge of the area and other brain regions are positive protein expression of c-fos, hippocampal tissue surrounding the expression level was significantly higher, which express the most significant cerebral contusion control group; sham-operated group only a few positive protein expression relevant doses of propofol can significantly inhibit c-fos protein expression in various brain regions, particularly in the hippocampus area around the most significant. Propofol uniform pumped into the group can significantly reduce the number of positive cells, the density of positive cells. Conclusion: cerebral contusion can induce the expression of c-fos gene, cerebral protective effect of propofol may be associated with down related to the abnormal expression of the c-fos gene.
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